This study was performed to answer the question whether extremely low frequency magnetic fields can affect in vitro DNA synthesis, DNA transcription or DNA repair and therefore exert an influence on carcinogenesis.
Bacteriophages, DNA mismatch repair-proficient human cervical carcinoma (HeLa) cells, and E.coli were used in the so called "DNA mismatch repair assay" as abnormalities in the DNA mismatch repair system have been regarded as one of the major cause of human cancer. DNA mismatch repair is a cellular system for recognising and repairing erroneous bases sequences that can arise during DNA replication and recombination, as-well as repairing some forms of DNA damage. In this study it should be investigated whether the exposure to extremely low frequency magnetic fields could affect the human cervical carcinoma cells ability to repair the base transition in the bacteriophage lacZ gene. Loss of DNA mismatch repair was visible in mixed coloured E.coli plaques.
|Exposure 1: 60 Hz|
Exposure duration: 15 min
|Setup||two iron cores, each with the shape of the letter E, whose two outside yokes were in contact and the middle yokes were separated by 13 mm and encircled by the coils; test tubes placed in the central gap of this field generator|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||1 T||peak value||measured||-||-|