Study type: Medical/biological study (experimental study)

Effects of high ELF magnetic fields on enzyme-catalyzed DNA and RNA synthesis in vitro and on a cell-free DNA mismatch repair. med./bio.

Published in: Bioelectromagnetics 2001; 22 (4): 260-266

Aim of study (acc. to author)

This study was performed to answer the question whether extremely low frequency magnetic fields can affect in vitro DNA synthesis, DNA transcription or DNA repair and therefore exert an influence on carcinogenesis.

Background/further details

Bacteriophages, DNA mismatch repair-proficient human cervical carcinoma (HeLa) cells, and E.coli were used in the so called "DNA mismatch repair assay" as abnormalities in the DNA mismatch repair system have been regarded as one of the major cause of human cancer. DNA mismatch repair is a cellular system for recognising and repairing erroneous bases sequences that can arise during DNA replication and recombination, as-well as repairing some forms of DNA damage. In this study it should be investigated whether the exposure to extremely low frequency magnetic fields could affect the human cervical carcinoma cells ability to repair the base transition in the bacteriophage lacZ gene. Loss of DNA mismatch repair was visible in mixed coloured E.coli plaques.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 1- 15 min for DNA synthesis; 1 - 30 min for RNA synthesis
Exposure 2: 60 Hz
Exposure duration: 15 min

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration 1- 15 min for DNA synthesis; 1 - 30 min for RNA synthesis
Exposure setup
Exposure source
Setup two iron cores, each with the shape of the letter E, whose two outside yokes were in contact and the middle yokes were separated by 13 mm and encircled by the coils; test tubes placed in the central gap of this field generator
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.25 T peak value measured - 0.25 T - 0.5 T
magnetic flux density 0.5 T peak value measured - -

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration 15 min
Additional info DNA mismatch repair
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 T peak value measured - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No statistically significant effects of the exposure to extremely low frequency magnetic field were observed in neither DNA/RNA synthesis nor DNA repair.

Study character:

Study funded by

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