To study the question whether in vivo exposure of rat leucocyte DNA to extremely low frequency electromagnetic fields leads to genetic effects associated with increased risk of cancer.
8-hydroxydeoxyguanosine assays (8OHdG) were used. 8OHdG is one of the predominant forms of radical-induced lesions to DNA and is a potential tool to asses the cancer risk.
To study the possible time-dependent changes resulting from magnetic field, 8-hydroxydeoxyguanosine (8OHdG) and thiobarbituric acid reactive substances (TBARS) were quantitated at 50 and 100 days.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
repeated daily exposure, 3 h/day for 50 days
|
|
|
Exposure 2:
50 Hz
Exposure duration:
repeated daily exposure, 3 h/day for 100 days
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | repeated daily exposure, 3 h/day for 50 days |
| Exposure source | |
|---|---|
| Chamber | methacrylate boxes (17 x 17 x 25 cm) |
| Setup | The animals were kept in 14/10 h light/dark environment at constant temperature of 22°C and 45% humidity. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 970 µT | mean | measured | - | ± 136 µT |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | repeated daily exposure, 3 h/day for 100 days |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 970 µT | mean | measured | - | ± 136 µT |
The data showed that the exposure to extremely low frequency electromagnetic fields induced oxidative DNA damage and lipid peroxidation.
The 8OHdG levels of exposed group were significantly higher than sham group at 50 and 100 days. The higher TBARS levels were also detected in the exposure group both on 50 and 100 days. In addition, the extent of DNA damage and lipid peroxidation would depend on the exposure time.
The results may have important implications for the long-term exposure to extremely low frequency electromagnetic fields which may cause oxidative DNA damage.
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