Study type: Medical/biological study (experimental study)

Oxidative DNA damage in rats exposed to extremely low frequency electro magnetic fields. med./bio.

Published in: Free Radic Res 2005; 39 (3): 317-323

Aim of study (acc. to author)

To study the question whether in vivo exposure of rat leucocyte DNA to extremely low frequency electromagnetic fields leads to genetic effects associated with increased risk of cancer.

Background/further details

8-hydroxydeoxyguanosine assays (8OHdG) were used. 8OHdG is one of the predominant forms of radical-induced lesions to DNA and is a potential tool to asses the cancer risk.
To study the possible time-dependent changes resulting from magnetic field, 8-hydroxydeoxyguanosine (8OHdG) and thiobarbituric acid reactive substances (TBARS) were quantitated at 50 and 100 days.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: repeated daily exposure, 3 h/day for 50 days
Exposure 2: 50 Hz
Exposure duration: repeated daily exposure, 3 h/day for 100 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration repeated daily exposure, 3 h/day for 50 days
Exposure setup
Exposure source
Chamber methacrylate boxes (17 x 17 x 25 cm)
Setup The animals were kept in 14/10 h light/dark environment at constant temperature of 22°C and 45% humidity.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 970 µT mean measured - ± 136 µT

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration repeated daily exposure, 3 h/day for 100 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 970 µT mean measured - ± 136 µT

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed that the exposure to extremely low frequency electromagnetic fields induced oxidative DNA damage and lipid peroxidation.
The 8OHdG levels of exposed group were significantly higher than sham group at 50 and 100 days. The higher TBARS levels were also detected in the exposure group both on 50 and 100 days. In addition, the extent of DNA damage and lipid peroxidation would depend on the exposure time.
The results may have important implications for the long-term exposure to extremely low frequency electromagnetic fields which may cause oxidative DNA damage.

Study character:

Study funded by

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