To evaluate whether exposure to cellular telephone fields (837 MHz microwaves) can increase TP53 protein level in human fibroblasts in vitro.
The expression level of the TP53 tumor suppressor protein (formerly named as p53) was chosen due to the fact that TP53 responds to a wide variety of environmental stressors.
|Exposure duration||continuous for 2 h|
|Chamber||Two 60 x 15-mm cell culture dishes (with 3 ml of culture medium) were placed side by side, on the central line in the direction of propagation, on each of the two shelves in the upper and lower chambers of a TEM cell (8 x 10 x 30 cm).|
|Setup||Power was coupled to the TEM cell through a power meter, and from the cell into a 600-W RF load.|
|Additional info||sham-exposure: same conditions, but without microwave exposure positive control: exposure to UV radiation (254 nm) for no more than 1 min to achieve 50 J/m² negative control: dishes were just left in the incubator|
The TP53 protein expression levels in cultured human fibroblast cells do not change significantly. Also the microscopic analysis revealed that human fibroblast did not show significant changes in the negative control cells, sham-irradiated cells, and microwave-irradiated cells.