Study type: Medical/biological study (experimental study)

Study of p53 expression and post-transcriptional modifications after GSM-900 radiofrequency exposure of human amniotic cells. med./bio.

Published in: Bioelectromagnetics 2013; 34 (1): 52-60

Aim of study (acc. to author)

The objective of this study was to investigate whether the exposure of a radiofrequency field of 900 MHz may induce the expression of the p53 protein and its activation by phosphorylation in cultured human amniotic cells.

Background/further details

Four average specific absorption rates were used to examine, if there is a change in the expression level of p53 protein and its phosphorylation of the serine residues 15 and 37. In response to a genetic damage, this protein is activated and can induce cell cycle arrest allowing more time for DNA repair or elimination of damaged cells through apoptosis. Cells were treated with Bleomycin as a positive control.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: pulsed
Exposure duration: continuous for 24 h
  • SAR: 4 W/kg average over time (0.25, 1, 2, 4 W/kg)
  • SAR: 32 W/kg peak value (2, 8, 16, 32 W/kg)

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Exposure duration continuous for 24 h
Modulation
Modulation type pulsed
Pulse width 0.577 ms
Repetition frequency 217 Hz
Exposure setup
Exposure source
Setup wire-patch cell placed in a box with absorbing ferrite walls; eight petri dishes exposed simultaneously in the wire-patch cell; one box for exposure and one for sham exposure placed in the same incubator
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 4 W/kg average over time - - 0.25, 1, 2, 4 W/kg
SAR 32 W/kg peak value - - 2, 8, 16, 32 W/kg

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:

Main outcome of study (acc. to author)

The data indicated no significant changes in the expression and activation of the p53 protein between exposed and sham-exposed cells. The authors suggest that a radiofrequency field exposure at 900 MHz does not induce the p53 pathway and cellular stress.

Study character:

Study funded by

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