研究のタイプ: 医学／生物学の研究 (experimental study)

[正常なヒト線維芽細胞でのTP53腫瘍抑制タンパクは837 MHzマイクロ波ばく露に応答しない] med./bio.

TP53 tumor suppressor protein in normal human fibroblasts does not respond to 837 MHz microwave exposure

著者: Li JR, Chou CK, McDougall JA, Dasgupta G, Wu HH, Ren RL, Lee A, Han J, Momand J
掲載誌: Radiat Res 1999; 151 (6): 710-716
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この研究は、TP53腫瘍サプレッサータンパク質（以前はp53として知られていた）のヒト線維芽細胞での応答が、837MHzマイクロ波ばく露の影響を受けるか否かを調べた。細胞は、温度制御されたTEMセル内で、837MHz連続波（CW）マイクロ波（SAR 0.9または9.0W / kg）の2時間ばく露を受けた。ばく露終了の2、8、24および48時間後に、ウエスタンブロット法でTP53タンパク質レベルを測定した。結果として、マイクロ波処理した細胞、擬似処理した細胞、および無処理の細胞におけるTP53タンパク質レベルは、試験したすべてにおいて互いに差がなかった；擬似処置細胞と比較して、マイクロ波処理細胞で形態学的変化は観察されなかった、と報告している。

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研究目的（著者による）

To evaluate whether exposure to cellular telephone fields (837 MHz microwaves) can increase TP53 protein level in human fibroblasts in vitro.
The expression level of the TP53 tumor suppressor protein (formerly named as p53) was chosen due to the fact that TP53 responds to a wide variety of environmental stressors.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 837 MHz
Modulation type: CW
ばく露時間: continuous for 2 h
  • SAR: 0.9 W/kg peak
  • SAR: 9 W/kg peak

ばく露1

主たる特性
周波数 837 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
チャンバの詳細 Two 60 x 15-mm cell culture dishes (with 3 ml of culture medium) were placed side by side, on the central line in the direction of propagation, on each of the two shelves in the upper and lower chambers of a TEM cell (8 x 10 x 30 cm).
ばく露装置の詳細 Power was coupled to the TEM cell through a power meter, and from the cell into a 600-W RF load.
Additional information sham-exposure: same conditions, but without microwave exposure positive control: exposure to UV radiation (254 nm) for no more than 1 min to achieve 50 J/m² negative control: dishes were just left in the incubator
パラメータ
測定量 値 種別 Method Mass 備考
SAR 0.9 W/kg peak 測定値および計算値 - -
SAR 9 W/kg peak 測定値および計算値 - -
測定および計算の詳細

Average and peak SAR were calculated from temperature increases in insulated culture dishes with 5.5 ml of culture medium. Four dishes were used for each exposure and a total of 56 exposures were averaged. According to the calculation of Guy et al. (see reference article) the peak SAR at the bottom of the culture dish (where the cells are located) is approx. three times the average SAR.

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The TP53 protein expression levels in cultured human fibroblast cells do not change significantly. Also the microscopic analysis revealed that human fibroblast did not show significant changes in the negative control cells, sham-irradiated cells, and microwave-irradiated cells.

研究の種別:

研究助成

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