Study type: Medical/biological study (experimental study)

Induction of increase in intracellular calcium concentration of embryonic cells and acceleration of morphogenetic cell movements during amphibian gastrulation by a 50-Hz magnetic field. med./bio.

Published in: J Exp Zool Part A Ecol Genet Physiol 2007; 307 (3): 156-162

Aim of study (acc. to author)

This in vivo study was performed to examine the influence of an extremely low frequency electromagnetic field on early development of amphibian embryos.

Background/further details

Additionally, the question whether electromagnetic field affects intracellular calcium concentration should be analyzed.
11 to 28 amphibian embryos per group were investigated in the development range from 2-cell-stage (or later) to the tail bud stage (after five days).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 5 days
Exposure 2: 50 Hz
Exposure duration: 5 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 5 days
Exposure setup
Exposure source
Chamber cylindrical plastic chamber with an inner diameter of 6 mm, 12 mm heigh
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT minimum measured - -
magnetic flux density 30 mT maximum measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 5 days
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT minimum measured - -
magnetic flux density 10 mT maximum measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Embryos exposed to the extremely low frequency electromagnetic field showed an accelerated rate of early development compared to control group. This effect of electromagnetic field was magnetic flux density dependent exerted preferentially at the gastrula stage, and the period of gastrulation was shortened.
Electromagnetic field increased the intracellular calcium concentration particularly in the cells isolated from gastrula.
No clear abnormality concerning morphological aspects in gastrulation was observed under the influence of electromagnetic field.
The data indicate that electromagnetic field specifically increased the intracellular calcium concentration of gastrula cells, thereby, accelerating the rate of cell development during gastrulation.

Study character:

Study funded by

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