Study type: Medical/biological study (experimental study)

EGF receptor clustering is induced by a 0.4 mT power frequency magnetic field and blocked by the EGF receptor tyrosine kinase inhibitor PD153035. med./bio.

Published in: Bioelectromagnetics 2007; 28 (3): 197-207

Aim of study (acc. to author)

To study the functional interaction of a 50 Hz, 0.4 mT magnetic field with a membrane receptor, epidermal growth factor receptor, mainly at the molecular level.

Background/further details

Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein expressed in many types of mammalian cell surfaces. An EGFR monomer contains an intracellular domain with tyrosine kinase which can be specifically inhibited by an EGFR tyrosine kinase-specific inhibitor PD153035 to block EGFR activation and to prevent intracellular signal pathway.
Purified EGFR (5 mg/ml) or cells were divided into three groups: 1) sham-exposed group, 2) magnetic field-exposed group, or 3) the PD inhibitor pretreated group with different concentrations.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 30 min

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 30 min
Exposure setup
Exposure source
  • a pair of circular horizontal Helmholtz coils, 20 cm radius, 20 cm gap between them, 150 turns
Setup The magnetic field was fairly uniform (± 0.012 mT) over the exposure area between the plates. The whole set-up was placed in a CO2 incubator maintained at 37°C and was shield from external field.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.4 mT unspecified measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed that the magnetic field exposure induced purified EGF receptors to form clusters (i.e. at least two monomers form a cluster), free from cell membrane and EGF binding. The EGFR tyrosine kinase inhibitor evidently blocks the ligand-free-clustering effect.
The findings indicate that the tyrosine kinase domain of EGFR is a likely candidate site for the magnetic field interaction.

Study character:

Study funded by

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