To study the effects of exposure to a 1.8 GHz radiofrequency electromagnetic field on epidermal growth factor receptor cluster formation (as initial process for signal transduction) and phosphorylation in human amniotic cells.
EGF treatment (100 ng/ml) served as positive control.
cells were treated in the following groups: i) positive control - treated with 100 ng/ml EGF ii) sham EMF exposure iii) EMF exposure
| Frequency | 1.8 GHz |
|---|---|
| Type | |
| Exposure duration | continuous for 15 min |
| Modulation type | pulsed |
|---|---|
| Pulse width | 0.576 ms |
| Duty cycle | 12.5 % |
| Repetition frequency | 217 Hz |
| Pulse type | rectangular |
| Exposure source | |
|---|---|
| Setup | waveguides placed inside an incubator with constant temperature of 37°C; six petri dishes with monolayers of cells placed on a dish holder in the H-field maximum of the standing wave inside the waveguide and were simultaneously exposed to E-polarization |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| SAR | 4 W/kg | maximum | - | - | 0.1, 0.5, 1, 2, 4 W/kg |
The data showed that, compared with sham exposure, radiofrequency exposure at specific absorption rate of 0.5, 1, 2, or 4 W/kg for 15 minutes significantly induced EGF receptor clustering and enhanced phosphorylation on the tyrosine-1173 residue in amniotic cells, whereas exposure to a SAR of 0.1 W/kg did not cause a significant effect. However, the results showed also that there was no additional significant increase in effects at intensities higher than 0.5 W/kg.
Based on these findings, the authors conclude that membrane receptors could be one of the main targets that radiofrequency exposure interacts with cells, and the dose-rate threshold, in the case of EGF receptors, is between SAR of 0.1 and 0.5 W/kg.
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