Study type: Medical/biological study (experimental study)

ELF non ionizing radiation changes the distribution of the inner chemical functional groups in human epithelial cell (HaCaT) culture. med./bio.

Published in: Electromagn Biol Med 2006; 25 (4): 281-289

Aim of study (acc. to author)

This in vitro study was performed to investigate changes in morphology and phospholipid/protein distribution of sinusoidal extremely low frequency electromagnetic field exposed human keratinocytes.

Background/further details

The temperature was continuously measured and maintained at the range of 37 +/- 0.3°C.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 24 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 24 h
Exposure setup
Exposure source
Setup solenoid with an inner diameter of 20 cm and a height of 40 cm consisting of 2 cm thick concrete-asbestos with 1.200 turns of 2 mm copper wire; exposure volume along the solenoid axis: 11 x 17 cm; temperature control
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -
electric field strength 0.01 µV/cm - calculated - -

Reference articles

  • Santoro N et al. (1997): Effect of extremely low frequency (ELF) magnetic field exposure on morphological and biophysical properties of human lymphoid cell line (Raji).
  • Stauffer PR et al. (1994): Practical induction heating coil designs for clinical hyperthermia with ferromagnetic implants
  • Ellinger DC et al. (1989): Evaluation of a semi-cylindrical solenoid as an applicator for radio-frequency hyperthermia.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Electromagnetic field exposed cells exhibited more flattened morphology compared to control group.
Exposed cells showed an increase of newly synthesized protein, jointly with a different phospholipid distribution.

Study character:

Study funded by

Related articles