Study type: Medical/biological study (experimental study)

Effects of GSM 1800 MHz on dendritic development of cultured hippocampal neurons. med./bio.

Published in: Acta Pharmacol Sin 2007; 28 (12): 1873-1880

Aim of study (acc. to author)

The effects of GSM modulated 1800 MHz microwaves exposure on development of dendrites in cultured hippocampal neurons in rats should be evaluated in this study.

Background/further details

Hippocampal neuronal primary cultures were extracted from newborn rats and investigated during neuronal development in the period from 6 days to 14 days in vitro (DIV6 to DIV14). The observations focused on morphological signs (dendritic filopodia, dendritic arborization, and dendritic spine maturation) which might indicate the efficiency of the synaptic network involved in spatial learning and memory.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1,800 MHz
Modulation type: pulsed
Exposure duration: repeated daily exposure, 15 min/day, for 9 days (DIV6 to DIV14)

Exposure 1

Main characteristics
Frequency 1,800 MHz
Type
Charakteristic
  • guided field
Exposure duration repeated daily exposure, 15 min/day, for 9 days (DIV6 to DIV14)
Modulation
Modulation type pulsed
Pulse width 0.576 ms
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional info

GSM signal

Exposure setup
Exposure source
Chamber Two waveguides, one for RF and one for sham exposure, were placed inside a conventional incubator at 37 ± 0.1 °C with 5% CO2 and 95% humidity. The temperature difference between both never exceeded 0.1 °C.
Setup Six 35-mm dishes were held by a dish holder exactly in the H field maximum of the standing wave and exposed simultaneously in E polarisation.
Sham exposure A sham exposure was conducted.
Additional info The system enabled the exposure of monolayer cells with a non-uniformity of less than 30%.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2.4 W/kg - - - -
SAR 0.8 W/kg - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

There was a significant decrease in the density and mobility of dendritic filopodia at day 8 in vitro and in the density of mature dendritic spines at day 14 in vitro in the neurons exposed to1800 MHz with 2.4 W/kg. In addition, the average length of dendrites per neuron at day 10 in vitro and day 14 in vitro was decreased, while the dendritic arborization was unaltered in these neurons.
However, there were no significant changes observed in the neurons exposed to the GSM 1800 MHz microwaves with 0.8 W/kg.
These results indicate that the chronic exposure to 2.4 W/kg GSM 1800 MHz microwaves during the early developmental stage may affect dendritic development and the formation of excitatory synapses of hippocampal neurons in culture.

Study character:

Study funded by

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