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Medical/biological study (experimental study)

Experimental evidence for involvement of nitric oxide in low frequency magnetic field induced obsessive compulsive disorder-like behavior.

Published in: Pharmacol Biochem Behav 2014; 122: 273-278

Aim of study (acc. to author)

To investigate the involvement of dopamine, serotonin and nitric oxide in extremely low frequency magnetic field-induced obsessive compulsive disorder-like behavior in mice.
Background/further details: Three experiments were performed:
In the first experiment, several groups of mice (n=8 per group) were subjected to behavioral tests. They were exposed or sham exposed for 7, 30, 60, 90 or 120 days. For the examination of the obsessive compulsive disorder-like behavior and for the determination of the locomotor activity, separate groups were employed.
In the second experiment, the levels of dopamine, serotonin and nitric oxide in the brain were determined after 120 days of sham exposure (n=8) or exposure (n=8).
In the third experiment, the influence of different nitric oxide modulators on behavior and levels of dopamine, serotonin and nitric oxide in the brain were examined. Mice were exposed or sham exposed for 120 days and received daily injections of saline solution (as a control), L-NAME (inhibitor of nitric oxide synthase; 15, 30 or 45 mg per kg body weight), L-ARG (nitric oxide precursor; 200, 400 or 800 mg per kg body weight) or 7-NI (inhibitor of nitric oxide synthase; 10, 20 or 40 mg per kg body weight) before the exposure/sham exposure. Separate groups (n=8 per group) were employed for each dose and examination.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 8 hours/day for 7, 30, 60, 90 or 120 days
Exposure 1
Main characteristics
Frequency 50 Hz
Type
Exposure duration 8 hours/day for 7, 30, 60, 90 or 120 days
Exposure setup
Exposure source
Chamber mice were housed in a clean plastic cage which was positioned on the center of the platform of the coils
Setup Helmholtz coils, spaced apart at a distance equal to their radii (45 cm); instrument was constructed by winding glaze-insulated copper wire (d = 1.2 mm) 100 times on wooden frames; both the coils were individually attached to regulated AC power supply through step down transformer (12 V) which acted as an isolator
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -
Measurement and calculation details
intensity of magnetic field measured with a digital gauss meter with Hall Effect Probe
Reference articles
  • Liu T et al. (2008): Anxiogenic effect of chronic exposure to extremely low frequency magnetic field in adult rats.
Exposed system:
  • animal
  • mouse/Swiss albino
  • whole body

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The first experiment revealed that magnetic field exposed mice buried significant more marbles than sham exposed mice. Furthermore, this effect was time dependent: The longer the mice were exposed the more marbles were buried and the more significant was the result. No significant differences between any of the groups were found regarding locomotor activity.
In the second experiment, no significant effect of the exposure on the levels of dopamine and serotonin in the brain of the mice was observed. However, exposure led to significantly increased nitric oxide levels in the cortex, hippocampus and hypothalamus when compared to the corresponding sham exposure.
In the third experiment, it was shown that the combination of the magnetic field exposure and the nitric oxide precursor L-ARG led to a further significant increase in the number of buried marbles compared to the magnetic field exposure alone, while the nitric oxide synthase inhibitors L-NAME and 7-NI significantly attenuated the exposure induced effect.
The authors conclude that exposure to extremely low frequency magnetic fields could induce obsessive compulsive disorder-like behavior in mice and that this effect appears to be associated with nitric oxide synthase activation.
Study character:

Study funded by

  • University Grants Commission (UGC), India

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