Study type: Medical/biological study (experimental study)

Exposure to low level GSM 935 MHZ radiofrequency fields does not induce apoptosis in proliferating or differentiated murine neuroblastoma cells. med./bio.

Published in: Radiat Prot Dosimetry 2008; 131 (3): 287-296

Aim of study (acc. to author)

This study was performed to examine whether radiofrequency fields characteristic of mobile phones at non-thermal levels can induce apoptosis in murine neuroblastoma cells in both proliferating and differentiated states.

Background/further details

The positive controls were X-ray (4 Gy) treated cells.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 935 MHz
Modulation type: pulsed
Exposure duration: continuous for 24 h
Exposure 2: 935 MHz
Modulation type: pulsed
Exposure duration: continuous for 24 h
Exposure 3: 935 MHz
Modulation type: CW
Exposure duration: continuous for 24 h

General information

The complete detail of the exposure apparatus, the signal profile, the exposure geometry and dosimetry have been described in [Schuderer et al., 2004].

Exposure 1

Main characteristics
Frequency 935 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 24 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional info

GSM basic

Exposure setup
Exposure source
Chamber Two identical waveguide cavities were placed in a tissue culture incubator maintained at 37°C with a humidified atmosphere of 5% CO2 in air. They were randomly assigned for RF or sham exposure. Good exposure and environmental control was achieved using field sensors, air temperature sensors, and an optimised airflow system.
Setup Cells were seeded into 35-mm Petri dishes in 3 ml of growth medium 24 h before any treatment and were placed inside the waveguides one hour before exposure. A specially designed holder allowed eight 35-mm Petri dishes to be placed accurately inside each chamber.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg mean - - -

Exposure 2

Main characteristics
Frequency 935 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 24 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional info

GSM talk (random changes between GSM basic and GSM DTX)

Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg mean - - -

Exposure 3

Main characteristics
Frequency 935 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 24 h
Modulation
Modulation type CW
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg mean - - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No statistically significant differences in apoptosis levels were found between the exposed and sham-exposed murine neuroblastoma cells neither in proliferating nor in differentiated state using the three assays at any time point post-radiofrequency irradiation.

Study character:

Study funded by

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