Study type: Medical/biological study (experimental study)

Does MW Radiation Affect Gene Expression, Apoptotic Level, and Cell Cycle Progression of Human SH-SY5Y Neuroblastoma Cells? med./bio.

Published in: Cell Biochem Biophys 2016; 74 (2): 99-107

Aim of study (acc. to author)

The effects of exposure of neuroblastoma cells to a 2.1 GHz electromagnetic field (W-CDMA) on apoptosis and cell cycle progression should be investigated.

Background/further details

Neuroblastoma cells were used as a in vitro model for neural cells in embryos and infants, as it is assumed that these developmental stages are more sensitive to electromagnetic fields than adults.



Exposure Parameters
Exposure 1: 2.1 GHz
Exposure duration: continuous for 24 hours

Exposure 1

Main characteristics
Frequency 2.1 GHz
Exposure duration continuous for 24 hours
Modulation type cf. additional info
Additional info

W-CDMA modulation

Exposure setup
Exposure source
Chamber polystyrene plate with 24 wells in a CO2 incubator
Setup antenna which radiated vertically upwards on the well plate 1 mm above; the temperature inside the incubator was kept constant at 37°C
Sham exposure A sham exposure was conducted.
Measurand Value Type Method Mass Remarks
electric field strength 55 V/m - measured - -
power 0.316 W - - - antenna input power
SAR 0.235 W/kg minimum calculated 1 g -
SAR 0.491 W/kg maximum calculated 1 g -
SAR 0.35 W/kg mean calculated 1 g -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The gene expressions of c-myc and cyclin D1 were significantly decreased and the percentage of cells in G1 phase was significantly higher in exposed cells compared to sham exposed cells, indicating cell cycle arrest in G1 phase.
There were no significant differences in apoptosis, cell viability or DNA fragmentation observed between exposed and sham exposed cells.
The authors conclude that exposure of neuroblastoma cells to a 2.1 GHz electromagnetic field (W-CDMA) has no effect on apoptosis but might change the cell cycle progression.

Study character:

Study funded by

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