Study type: Medical/biological study (experimental study)

Terahertz radiation induces spindle disturbances in human-hamster hybrid cells. med./bio.

Published in: Radiat Res 2011; 175 (5): 569-574

Aim of study (acc. to author)

To study and quantify the production of spindle disturbances at the anaphase and telophase in human hamster-hybrid cells by 0.106 THz radiation.

Background/further details

For each exposure protocol, two separate replicate experiments were performed.
The aneuploidy-inducing insecticide trichlorfon was used as positive control.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 0.106 THz
Modulation type: CW
Exposure duration: continuous for 0.5 h

Exposure 1

Main characteristics
Frequency 0.106 THz
Type
Exposure duration continuous for 0.5 h
Modulation
Modulation type CW
Exposure setup
Exposure source
Setup cells placed in µ-dishes (Petri dishes) in an incubator at 36°C and 5 % CO2; incubator fitted with a transparent window on one side and a metallic mirror for transmission of the radiation beam; gaussian beam directed from the horn antenna through a mirror and the incubator's window on a second mirror that directed the beam from below on the cell dishes; exposure area with a diameter of 12 mm
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power density 0.043 mW/cm² spatial average measured - -
power density 0.43 mW/cm² spatial average measured - -
power density 4.3 mW/cm² spatial average measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Based on a total of 6,365 analyzed mitotic cells, the data of two replicate experiments suggest that 0.106 THz radiation at power density levels of 0.43 and 4.3 mW/cm² is a spindle-acting agent as predominately indicated by the appearance of spindle disturbances at the anaphase and telophase (especially lagging and non-disjunction of single chromosomes) of cell divisions.
The findings do not necessarily imply disease or injury but may be important for evaluating possible underlying mechanisms.

Study character:

Study funded by

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