Study type: Medical/biological study (experimental study)

Confirmation studies of Soviet research on immunological effects of microwaves: Russian immunology results. med./bio.

Published in: Bioelectromagnetics 2010; 31 (8): 589-602

Aim of study (acc. to author)

To confirm previous data on the effects of 2450 MHz microwave exposure on immunological processes and teratology in rats.

Background/further details

A series of Soviet publications published from 1974-1986 (Vinogradov et al. 1974, Vinogradov et al. 1975, Shandala et al. 1983, Vinogradov et al. 1985, Vinogradov and Naumenko 1986, Shandala and Vinogradov 1982) dealt with the effects on the immune system of rats exposed to radiofrequency fields at 2375 MHz (0.1-10 W/m²). Because the findings of these studies served in part as the basis for setting exposure limit values in the former USSR, it was deemed necessary to perform confirmation studies using modern dosimetric and biological methods.
In the previous studies it was reported that radiofrequency exposure disrupted the antigenic structure of rat brain tissue and induced differences in immune responses. Moreover, these studies showed that blood serum from irradiated rats injected into intact non-exposed female rats on the 10th day of pregnancy led to increased postimplantation embryo mortality and decreased fetus body weight and size.
For immunological investigation blood, brain and liver of 48 rats (16 rats/group; sham exposure, exposure, cage control) was taken on days 7 (5 rats per group) and 14 (11 rats per group) after exposure.
For teratological experiments, blood sera of the 11 rats (14 days following exposure) were prepared. Sera from exposed and sham exposed rats were administered to two groups of female rats on the 10th day of pregnancy (21 rats/group). A third group of pregnant females received no treatment (control group).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2,450 MHz
Exposure duration: continuous for 7 hr/day, 5 days/week for 30 days
  • SAR: 0.16 W/kg (at 5 W/m²)
  • SAR: 0.16 W/kg average over mass (brain)
  • SAR: 9.9 W/kg peak value (partial body) (tail skin)
  • SAR: 1 W/kg peak value (brain)
  • power density: 4.95 W/m² average over time (3.12 W/m² - 7.82 W/m² at the geometric center of the cages measured 0.22 m above the floor in the absence of animals and cages)
  • power: 71 W (+/- 7.3 W antenna input power)
  • power density: 5 W/m² (incident power in the cage)

Exposure 1

Main characteristics
Frequency 2,450 MHz
Type
Charakteristic
Polarization
  • elliptical
Exposure duration continuous for 7 hr/day, 5 days/week for 30 days
Additional info +/- 50 MHz
Exposure setup
Exposure source
  • magnetron + helical antenna with an external diameter of 90 mm
Distance between exposed object and exposure source 2.35 m
Chamber 6 m x 3 m x 3.5 m anechoic chamber with walls, floor and ceiling covered with 0.05 m high ferrite-based pyramidal RF-absorber elements; outer walls of the chamber covered with welded steel sheets; antenna mounted on a bracket made of plastic and wood above the chamber's floor
Setup 16 cages made of Plexiglas and PVC arranged in a ring inside the chamber with one rat per cage; ring of cages placed on 0.18 m high styrofoam stands
Sham exposure A sham exposure was conducted.
Additional info chamber 1 for the sham-exposed animals was 10 m x 3 m x 3.5 m, and chamber 2 for RF-exposed animals was 6 m x 3 m x 3.5 m; to make the chambers visually the same size, a black opaque curtain was put in chamber 1
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.16 W/kg - estimated whole body at 5 W/m²
SAR 0.16 W/kg average over mass estimated brain -
SAR 9.9 W/kg peak value calculated partial body tail skin
SAR 1 W/kg peak value calculated brain -
power density 4.95 W/m² average over time measured - 3.12 W/m² - 7.82 W/m² at the geometric center of the cages measured 0.22 m above the floor in the absence of animals and cages
power 71 W - measured - +/- 7.3 W antenna input power
power density 5 W/m² - measured - incident power in the cage

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

The data of the immunological examination partly confirmed the results of the Soviet research groups on the possible induction of autoimmune responses (increase of antibodies against brain antigens in the exposed group on the 14th day after exposure) and suggested stress reactions from radiofrequency exposure on day 7 after exposure (indicated by an increase in the content of amino acids bound with nitric oxide compounds, as well as lipids, including non-saturated fatty acids with short chains and their oxidation products).
The findings on prenatal development of offspring suggested possible adverse effects of the blood serum from exposed rats on pregnancy and embryo-foetal development in rats, in agreement with the earlier results of Shandala and Vinogradov (1982). The present study showed higher total in utero mortality (pooled data of days 15 and 20 of pregnancy) in rats injected with blood serum from exposed animals (55.6≥4.0%) than in rats injected with serum from sham exposed animals (11.7≥3.3%).
The application of these results in developing exposure standards is limited.

Study character:

Study funded by

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