Study type: Medical/biological study (experimental study)

Potential protection of green tea polyphenols against 1800 MHz electromagnetic radiation-induced injury on rat cortical neurons. med./bio.

Published in: Neurotox Res 2011; 20 (3): 270-276

Aim of study (acc. to author)

To study the possible protective effects of green tea polyphenols against electromagnetic irradiation-induced injury in the cultured rat cortical neurons.

Background/further details

After exposure, the cells were treated with green tea polyphenols for 4 h (different concentrations). Green tea polyphenols have been shown to have a antioxidant capacity, anti-proliferative ability, and anti-carcinogenic properties in neurodegenerative diseases.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1,800 MHz
Exposure duration: continuous for 6 x 2 h/day on 2 days

Exposure 1

Main characteristics
Frequency 1,800 MHz
Type
Exposure duration continuous for 6 x 2 h/day on 2 days
Exposure setup
Exposure source
Chamber incubator with a constant temperature of 37°C, 95% air and 5% CO2
Setup mobile phone placed above the cell culture plates with the antenna positioned over their center
Sham exposure A sham exposure was conducted.
Additional info for sham exposure the mobile phone was in stand-by mode
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 28.67 V/m minimum - - +/- 0.88 V/m in the cell culture plates
electric field strength 92.67 V/m minimum - - +/- 1.2 V/m in the cell culture plates

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed that the mobile phone exposure for 24 h induced marked neuronal cell death in the TUNEL assay and a decreased cell viability in the MTT assay. Protective effects of green tea polyphenols (10 µM) on the injured cortical neurons were demonstrated by testing the content of Bax. The mobile phone irradiation-induced increases in the content of active Bax were inhibited significantly by green tea polyphenols, while the contents of total Bax had no marked changes after the treatment of green tea polyphenols. The mobile phone exposure increased the protein expression of Hsp70 compared with the sham exposed control.
The results suggest a neuroprotective effect of green tea polyphenols against the mobile phone irradiation-induced injury in the cultured rat cortical neurons.

Study character:

Study funded by

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