Study type: Medical/biological study (experimental study)

Spindle disturbances in human-hamster hybrid (AL) cells induced by mobile communication frequency range signals. med./bio.

Published in: Bioelectromagnetics 2008; 29 (8): 626-639

Aim of study (acc. to author)

To evaluate in more detail (in comparison to a previous study, cf. publication 16115) the capacity of radiofrequency irradiation at a electric field strength of 90 V/m to act during a narrow time dependent window of the mitotic cycle, that is, the passage from metaphase to anaphase.

Background/further details

In the preliminary study on the induction of spindle disturbances in mammalian cells by radiofrequency exposure, the authors could demonstrate that with increasing electric field strength from 20 to 90 V/m there was an obvious tendency for an increase in the fraction of damaged anaphases and telophases but a less marked increase in the fraction of damaged metaphases.
Positive controls were exposed to trichlorfon.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 835 MHz
Modulation type: CW
Exposure duration: continuous for 0, 0.17, 0.5, 1, and 2 h

Exposure 1

Main characteristics
Frequency 835 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 0, 0.17, 0.5, 1, and 2 h
Modulation
Modulation type CW
Exposure setup
Exposure source
Chamber A special exposure setup was used [Schmid and Schrader, 2007] that fulfilled the minimal requirements proposed by [Kuster and Schonborn, 2000] and allowed optimal experimental conditions for small biological samples. The µTEM cell itself was optimized to provide a very low VSWR close to unity, and standing waves were avoided as much as possible by the chosen setup.
Setup The FC2 cells were grown as a monolayer in so-called slide flasks and were exposed at room temperature (20-22 °C) in a µTEM cell.
Additional info Positive controls were exposed to trichlorfon for up to 6 h.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 90 V/m effective value calculated - -
SAR 60 mW/kg maximum calculated - -

Reference articles

  • Schmid E et al. (2007): Different biological effectiveness of ionising and non-ionising radiations in mammalian cells.
  • Kuster N et al. (2000): Recommended minimal requirements and development guidelines for exposure setups of bio-experiments addressing the health risk concern of wireless communications.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data suggest that an electric field strength of 90 V/m is a spindle acting agent in human-hamster hybrid cells as indicated by the appearance of spindle disturbances at the metaphase stage and preferably at the anaphase and telophase stages of cell divisions. The fraction of anaphases and telophases with spindle disturbances increased with increasing exposure time from a low spontaneous fraction to a saturation value of 20%.
It may be possible that electromagnetic fields induce biological effects in FC2 cells via non-thermal pathways. However, this finding does not necessarily mean that the induced effects lead to disease.

Study character:

Study funded by

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