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Medical/biological study (experimental study)

In vitro effects of radiofrequency electromagnetic waves on bovine spermatozoa motility.

Published in: J Environ Sci Health A Tox Hazard Subst Environ Eng 2011; 46 (12): 1417-1423

Aim of study (acc. to author)

To study the possible effects of radiofrequency electromagnetic fields on bovine spermatozoa motility parameters in relation to exposure duration.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1,800 MHz
Exposure duration: continuous for 30, 120 or 420 min
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Exposure 1
Main characteristics
Frequency 1,800 MHz
Type
Exposure duration continuous for 30, 120 or 420 min
Exposure setup
Exposure source
  • not specified
Setup samples placed in micro-titre plates with wells of 400 µm; plates positioned in an incubator at 37°C, which contained a microwave source
Parameters
No parameters are specified for this exposure.

Methods Endpoint/measurement parameters/methodology

  • sperm motility, progressive sperm motility, different sperm parameters concerning the covered path and velocity of the sperms: curvilinear distance (DCL), distance average path (DAP), distance straight line (DSL), curvilinear velocity line (VCL), velocity average path (VAP), velocity straight line (VSL), linearity, amplitude of lateral head displacement (ALH), beat cross frequency; computer assisted semen analyzer (CASA)
Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The evaluation of the percentage of motile spermatozoa showed a significant decrease in exposed samples after 120 and 420 minutes of exposure compared with the sham exposed samples. Similar spermatozoa motility inhibition was detected for the percentage of progressively motile spermatozoa, too.
Average path distance (DAP) decreased significantly in the exposed samples after 30 and 420 minutes of exposure. Path velocity parameters (VAP, VCL, VSL) increased in the irradiated spermatozoa samples after 30 minutes of exposure, but subsequently decreased after 420 minutes of exposure compared with the control. This indicates a possible initial stimulation and subsequent velocity inhibition of bovine spermatozoa under radiofrequency electromagnetic field exposure.
A significant decrease was revealed for amplitude of lateral head displacement in the exposed spermatozoa samples after 420 minutes of exposure.
The detailed in vitro sperm motility analysis of bovine spermatozoa exposed to microwaves suggested that the parameters of the covered path and velocity of the sperms at the beginning of exposure significantly increased, but after longer exposure (420 minutes) a significant decrease occured in the irradiated samples compared with the control. In general, the data indicate a negative time-dependent effect of 1800 MHz radiofrequency electromagnetic field exposure on bovine sperm motility.
Study character:

Study funded by

  • Ministry of Education, Slovakia

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