Semen samples were collected from healthy, nonsmoking donors (n=12) by masturbation after 2-3 days of sexual abstinence. The semen parameters of these samples were within the normal range of the WHO criteria. Samples were exposed to two different specific absorption rates (2.0 and 5.7 W/kg) and run in duplicate.
The sperm motility was assessed by objective motility measurement and by the inner mitochondrial membrane potential as its decrease is highly correlated with diminished sperm motility and fertilisation potential.
|Exposure duration||continuous for 1 h|
|Pulse width||0.577 ms|
|Repetition frequency||217 Hz|
|Chamber||The exposure chamber was positioned vertically inside a CO2 incubator.|
|Setup||Two 55-mm glass Petri dishes were placed inside the chamber on a 9-mm thin rectangular glassfibre-moulded, temperature controlled waterbed. The surface of the culture medium was aligned parallel to the E-field vector.|
|Additional info||Cell temperatures measured during exposure ranged from 36.7 to 37.3 °C at 5.7 W/kg and from 36.8 to 37.2 °C at 2 W/kg.|
There was no effect of pulsed 900 MHz GSM irradiation on the mitochondrial membrane potential. Additionally, no changes for all kinematic sperm motility parameters assessed at a specific absorption rate of 2.0 W/kg were observed. However, two kinematic parameters of sperm's velocity and motion were significantly decreased after the exposure at the specific absorption rate of 5.7 W/kg.
Further research is relevant to clarify whether the observed effects at the specific absorption rate of 5.7 W/kg are artefacts or cellular mechanisms verifiable.