Study type: Medical/biological study (experimental study)

Non-thermal activation of the hsp27/p38MAPK stress pathway by mobile phone radiation in human endothelial cells: Molecular mechanism for cancer- and blood-brain barrier-related effects. med./bio.

Published in: Differentiation 2002; 70 (2-3): 120-129

Aim of study (acc. to author)

To determine whether non-thermal exposure of cells to 900 MHz GSM mobile phone radiation activates signal transduction pathways and induces cellular stress response in a human model.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: pulsed
Exposure duration: continuous for 1 h

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 1 h
Modulation
Modulation type pulsed
Pulse width 0.577 ms
Repetition frequency 217 Hz
Exposure setup
Exposure source
Chamber The irradiation chamber was placed vertically inside a cell culture incubator.
Setup The modulated RF signal was amplified and fed to the exposure waveguide via a monopole type feed post. Two 55 mm glass Petri dishes were placed on a thin (9 mm) rectangular glass-fiber-molded temperature-controlled waterbed at the bottom of the chamber in such a way that the electric field vector was parallel to the plane of the culture medium.
Additional info The only difference between irradiated and sham samples was that the RF-EMF radiation was either turned on or turned off.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg mean measured and calculated - 1.8-2.5 W/kg

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The results suggest that mobile phone radiation activates cellular signal transduction and stress response pathways. 1-hour of non-thermal exposure of the cells changes phosphorylation status of numerous, yet largely unidentified, proteins. One of these proteins was heat shock protein 27 (hsp27). Mobile phone exposure caused a transient increase in phosphorylation of hsp27. This was prevented by a specific inhibitor of p38 mitogen-activated protein kinase (p38MAPK). Mobile phone caused also transient changes in the protein expression levels of p38MAPK and hsp27.

Study character:

Study funded by

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