研究のタイプ: 医学／生物学の研究 (experimental study)

[1.8 GHzの無線周波放射は培養したヒト羊膜（FL）細胞における上皮成長因子（EGF）受容体のクラスタリングとリン酸化を誘導する] med./bio.

A 1.8-GHz radiofrequency radiation induces EGF receptor clustering and phosphorylation in cultured human amniotic (FL) cells

著者: Sun W, Shen X, Lu D, Fu Y, Lu D, Chiang H
掲載誌: Int J Radiat Biol 2012; 88 (3): 239-244
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この研究では、比吸収率（SAR）が0.1から4.0W/kgの1.8GHzの無線周波放射（RFR）への15分間のばく露が、ヒト羊膜（FL）細胞における上皮成長因子（EGF）受容体のクラスタリングとリン酸化に対する影響を調べた。その結果から、膜受容体はRFRが細胞と相互作用する主なターゲットの一つになり得るかも知れないこと、またEGF受容体の場合、ばく露量率の閾値は0.1と0.5W/kgの間にある、と結論している。

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研究目的（著者による）

To study the effects of exposure to a 1.8 GHz radiofrequency electromagnetic field on epidermal growth factor receptor cluster formation (as initial process for signal transduction) and phosphorylation in human amniotic cells.

詳細情報

EGF treatment (100 ng/ml) served as positive control.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1.8 GHz
Modulation type: pulsed
ばく露時間: continuous for 15 min
  • SAR: 4 W/kg maximum (0.1, 0.5, 1, 2, 4 W/kg)

General information

cells were treated in the following groups: i) positive control - treated with 100 ng/ml EGF ii) sham EMF exposure iii) EMF exposure

ばく露1

主たる特性
周波数 1.8 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 15 min
Modulation
Modulation type pulsed
Pulse width 0.576 ms
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 waveguides placed inside an incubator with constant temperature of 37°C; six petri dishes with monolayers of cells placed on a dish holder in the H-field maximum of the standing wave inside the waveguide and were simultaneously exposed to E-polarization
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 4 W/kg maximum - - 0.1, 0.5, 1, 2, 4 W/kg
Additional parameter details

background field: 40 - 60 nT

Reference articles

  • Diem E et al. (2005): [イン・ビトロでのヒト線維芽細胞および形質転換GFSH-R17ラット顆粒膜細胞における携帯電話放射（1800MHz）による非熱的なDNA切断]
  • Schönborn F et al. (2001): [移動通信の健康ハザード評価にためのIn vitroばく露装置の最適化]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The data showed that, compared with sham exposure, radiofrequency exposure at specific absorption rate of 0.5, 1, 2, or 4 W/kg for 15 minutes significantly induced EGF receptor clustering and enhanced phosphorylation on the tyrosine-1173 residue in amniotic cells, whereas exposure to a SAR of 0.1 W/kg did not cause a significant effect. However, the results showed also that there was no additional significant increase in effects at intensities higher than 0.5 W/kg.
Based on these findings, the authors conclude that membrane receptors could be one of the main targets that radiofrequency exposure interacts with cells, and the dose-rate threshold, in the case of EGF receptors, is between SAR of 0.1 and 0.5 W/kg.

研究の種別:

研究助成

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