Study type: Medical/biological study (experimental study)

Investigation of transcriptional responses of juvenile mouse bone marrow to power frequency magnetic fields. med./bio.

Published in: Mutat Res Genet Toxicol Environ Mutagen 2013; 745: 40-45

Aim of study (acc. to author)

To examine changes in gene expression in bone marrow cells of in vivo exposed juvenile mice to power frequency magnetic fields.

Background/further details

A pilot study with 6 sham exposed and 6 exposed mice identified four candidate transcripts as a response to the magnetic field by using two methods (HiCEP and real-time RT-PCR). In a larger experiment, 27 mice were exposed and 24 mice were sham exposed. Only samples with a good RNA quality were used for examinations. Using microarray and real-time RT-PCR, 19 samples of exposed and 15 samples of sham exposed mice were compared with regard to transcriptional changes.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 2 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 2 hours
Exposure setup
Exposure source
Chamber in the pilot study, groups of 3 mice were placed within the coils in octagonal (?) acrylic exposure cages (26 cm Π26 cm Π25 cm high); in the larger experiment, groups of 3 mice were placed within the coils in square, acrylic cages (30 cm Π30 cm Π14 cm)
Setup a pair of horizontal Helmholtz coils was used (inner radius of 25.5 cm), consisting of 150 turns of 1 mm diameter copper wire wound on a frame made from Tufnol resin; an earthed screen was wound around both coils to prevent stray electric fields
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant changes in the gene expression between exposed and sham exposed mice were found.
This study provides no evidence that exposure to a 50 Hz magnetic field affects gene expression.

Study character:

Study funded by

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