A pilot study with 6 sham exposed and 6 exposed mice identified four candidate transcripts as a response to the magnetic field by using two methods (HiCEP and real-time RT-PCR). In a larger experiment, 27 mice were exposed and 24 mice were sham exposed. Only samples with a good RNA quality were used for examinations. Using microarray and real-time RT-PCR, 19 samples of exposed and 15 samples of sham exposed mice were compared with regard to transcriptional changes.
Exposure duration: continuous for 2 hours
|Chamber||in the pilot study, groups of 3 mice were placed within the coils in octagonal (?) acrylic exposure cages (26 cm Œ 26 cm Œ 25 cm high); in the larger experiment, groups of 3 mice were placed within the coils in square, acrylic cages (30 cm Œ 30 cm Œ 14 cm)|
|Setup||a pair of horizontal Helmholtz coils was used (inner radius of 25.5 cm), consisting of 150 turns of 1 mm diameter copper wire wound on a frame made from Tufnol resin; an earthed screen was wound around both coils to prevent stray electric fields|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||100 µT||-||measured||-||-|