To examine changes in gene expression in bone marrow cells of in vivo exposed juvenile mice to power frequency magnetic fields.
A pilot study with 6 sham exposed and 6 exposed mice identified four candidate transcripts as a response to the magnetic field by using two methods (HiCEP and real-time RT-PCR). In a larger experiment, 27 mice were exposed and 24 mice were sham exposed. Only samples with a good RNA quality were used for examinations. Using microarray and real-time RT-PCR, 19 samples of exposed and 15 samples of sham exposed mice were compared with regard to transcriptional changes.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
continuous for 2 hours
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 2 hours |
| Exposure source | |
|---|---|
| Chamber | in the pilot study, groups of 3 mice were placed within the coils in octagonal (?) acrylic exposure cages (26 cm Π26 cm Π25 cm high); in the larger experiment, groups of 3 mice were placed within the coils in square, acrylic cages (30 cm Π30 cm Π14 cm) |
| Setup | a pair of horizontal Helmholtz coils was used (inner radius of 25.5 cm), consisting of 150 turns of 1 mm diameter copper wire wound on a frame made from Tufnol resin; an earthed screen was wound around both coils to prevent stray electric fields |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 100 µT | - | measured | - | - |
No significant changes in the gene expression between exposed and sham exposed mice were found.
This study provides no evidence that exposure to a 50 Hz magnetic field affects gene expression.
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