Study type: Medical/biological study (experimental study)

Extremely low frequency magnetic field induces human neuronal differentiation through NMDA receptor activation. med./bio.

Published in: J Neural Transm (Vienna) 2019; 126 (10): 1281-1290

Aim of study (acc. to author)

The effects of exposure of human stem cells to a 50 Hz magnetic field on neuronal differentiation and the involvement of the NMDA receptor should be investigated.

Background/further details

Cells were divided into an exposure group and a sham exposure group. To investigate the role of the NMDA receptor, the cells were partially treated with memantine, an NMDA receptor antagonist.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 5 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 5 days
Exposure setup
Exposure source
Setup Helmholtz coil pair placed inside a cell culture incubator; power supply signal was attenuated by a variac and fed to the coils to produce magnetic field
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -

Reference articles

  • Cho H et al. (2012): Neural stimulation on human bone marrow-derived mesenchymal stem cells by extremely low frequency electromagnetic fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Both immunohistochemical staining and Western blot showed a significant increase in neuronal differentiation of cells in the exposure group compared to the sham exposure group. The differentiated neurons in the exposure group also showed significantly longer axons and a significantly increased level of c-Fos compared to the sham exposure group. Addition of the NMDA receptor inhibitor memantine reversed these effects.
The authors conclude that exposure of human stem cells to a 50 Hz magnetic field could promote neuronal differentiation with the involvement of the NMDA receptor.

Study character:

Study funded by

Related articles