|Chamber||10 cm-cell culture dishes|
|Setup||2 Merritt coils were placed side-by-side in a CO2 incubator; each Merritt coil comprised four sub-coils, 2 electrical wires were wound in parallel around each sub-coil; each Merritt coil generated the same amount of Joule heat, and the temperature difference between the exposure and sham exposure coils was minimized (0.2°C difference between samples of exposure and sham exposure); a sheet of magnetic shielding was placed between exposure and sham exposure coils to reduce stray fields; in each coil, 4 cell culture dishes were placed on a rack made of acrylic plastic|
|Sham exposure||A sham exposure was conducted.|
|Additional info||in the sham exposure coil, electrical current flowed through each of the two wires in opposite directions so the magnetic fields generated by each wire canceled each other|
|magnetic flux density||100 µT||-||measured||-||-|
A total of 43 genes showed a significantly different and considerable, i.e. at least doubled or halved, gene expression in exposed cells compared to the sham exposure. 20 of these genes were upregulated, 23 were downregulated. However, after correction for multiple testing, the results were no longer significant, leaving open the possibility that the altered expression of these transcripts was a false-positive result.
The authors conclude that the effects of exposure of human umbilical vein endothelial cells to a 50-Hz magnetic field on the global gene expression profile could not be clarified within the study.