The results of the magnetic field exposure were compared to the effects of X-radiation (50 cGy).
In total, 168 mice were used. For the magnetic field exposures as well as for the X-radiation, 14 groups were examined, resepctively (n=6 per group).
In the first experiment, mice were exposed to the magnetic field for 7, 14, 21 or 28 days and killed one day after the last exposure. The control group without magnetic field exposure was kept for 28 days. Four antioxidants (dimethyl sulfoxide (DMSO), 6-n-propyt-2-thiouracil (PTU), grape procyanidins (P), citrus flavonoids extract (CE)) were administered to try to reduce the chromosomal damage. These substances were given for 28 days. Furthermore, control groups without exposure which received the substances for 28 days were examined.
In a second experiment, mice were treated with X-radiation. The same antioxidants as in the magnetic field exposure groups were administered 5 days before or 1 day after the radiation. Additional control groups without X-radiation but with the antioxidants were examined.
Exposure duration: continuous for 7, 14, 21 or 28 days
|Exposure duration||continuous for 7, 14, 21 or 28 days|
|magnetic flux density||200 µT||-||measured||-||± 20 µT|
The bone marrow of magnetic field exposed mice (p value < 0.01) and of X-radiated mice (p value < 0.001) showed a significantly increased number of micronucleated erythrocytes in comparison to the corresponding control group. In the magnetic field exposed groups, an administration of the antioxidants did not significantly influence the number of micronuclei. However, groups that were treated with X-radiation and antioxidants had significantly decreased numbers of micronuclei compared to X-radiation alone.
The authors conclude that the extremely low frequency magnetic field had a genotoxic effect in mouse bone marrow and that the administered antioxidants could not prevent this effect.