Study type: Medical/biological study (experimental study)

Cytogenetic effects of extremely low frequency magnetic field on Wistar rat bone marrow. med./bio.

Published in: Mutat Res Genet Toxicol Environ Mutagen 2007; 630 (1-2): 69-77

Aim of study (acc. to author)

The potential genotoxic and cytotoxic effects of acute (for four hours) and long-term exposure (for 45 days) to 50 Hz magnetic field on tibia bone marrow cells of rats in vivo should be investigated.

Background/further details

The experiment was carried out with healthy adult female Wistar rats (eight weeks of age, with average body weight of 178±15 g).
The positive control consisted of animals treated with mitomycin C (2 mg/kg body weight).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: experiment 1: 4 h continuous; experiment 2: 4 h/day for 45 days

General information

rats were treated in four groups: i) short time exposure to EMF ii) long time exposure to EMF iii) sham exposure iv) positive control: injection of 2 mg/kg Mitomycin C

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration experiment 1: 4 h continuous; experiment 2: 4 h/day for 45 days
Exposure setup
Exposure source
Setup Helmholtz coil system consisting of coils with 42.75 cm diameter and a clearance of 21.375 cm, made of 160 turns of insulated copper wire; 26 x 17 x 13 cm polycarbonate cages in the center of the coils
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - +/- 0.05 mT

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The results concerning genotoxicity revealed no statistically significant differences between the negative control and the exposure groups in chromosome aberrations. However, a statistically significant higher mean micronucleus frequency was observed in the long-term magnetic field exposed group compared to the negative control and acutely magnetic field exposed group.
The mitotic index in bone marrow of both magnetic field exposed groups was significantly decreased when compared to that in the negative control. Additionally, bone marrow cytotoxicity (depression) was indicated by a significant lower ratio of polychromatic erythrocytes to normochromatic erythrocytes in both magnetic field exposed groups.
These results indicate that the exposure to extremely low frequency magnetic field may be genotoxic and cytotoxic on bone marrow cells of mammals.

Study character:

Study funded by

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