Study type: Medical/biological study (experimental study)

Evaluation of genotoxic effects in human fibroblasts after intermittent exposure to 50 Hz electromagnetic fields: a confirmatory study. med./bio.

Published in: Radiat Res 2005; 164 (3): 270-276

Aim of study (acc. to author)

To confirm the results of two recent studies (see publication 9990 and publication 9089) on the induction of genotoxic effects in human fibroblasts exposed to 50 Hz intermittent (5 min field on/10 min field off) sinusoidal electromagnetic fields.

Background/further details

To extend the investigation and validate the data, in the same experimental conditions, the potential genotoxicity was also tested by exposing the cells to a 50 Hz powerline signal.
Positive controls were also provided by treating fibroblasts with hydrogen peroxide or mitomycin C for the comet assay or micronucleus assay, respectively.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: intermittent, 5 min field on/10 min field off for 15 or 24 h
Exposure 2: 50 Hz
Exposure duration: intermittent, 5 min field on/10 min field off for 15, 18 or 24 h
power line signal consisting of 50 Hz fundamental frequency as well the harmonics.

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent, 5 min field on/10 min field off for 15 or 24 h
Additional info pure sinusoidal signal
Additional info pure sinusoidal signal
Exposure setup
Exposure source
Setup Exposure system consisted of two identical apparatuses made of four coaxial square coils (20 cm long) placed horizontally hence parallel to the surface of the culture plates, each of them housed in a µ-metal shielding box in the region of uniform MF (1% for 12 x 12 x 20 cm³ region and 5% within a region of 15 x 15 x 20 cm³). Coils were connected wound or counter wound configurations. The latter was used for sham exposure.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent, 5 min field on/10 min field off for 15, 18 or 24 h
Additional info power line signal consisting of 50 Hz fundamental frequency as well the harmonics.
Additional info Powerline signal consisting of 50 Hz fundamental frequency as well the harmonics.
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -

Reference articles

  • Ivancsits S et al. (2002): Induction of DNA strand breaks by intermittent exposure to extremely-low-frequency electromagnetic fields in human diploid fibroblasts.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No statistically significant difference was found in exposed compared to sham-exposed samples in any of the experimental conditions tested. In contrast, the positive controls showed a statistically significant increase in DNA damage in all cases, as expected.
Accordingly, the results do not confirm the findings reported previously for either comet induction or an increase in micronucleus frequency.

Study character:

Study funded by

Replicated studies

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