Study type: Medical/biological study (experimental study)

Effects of long-term 50 Hz magnetic field exposure on the micro nucleated polychromatic erythrocyte and blood lymphocyte frequency and argyrophilic nucleolar organizer regions in lymphocytes of mice. med./bio.

Published in: Neuro Endocrinol Lett 2010; 31 (2): 208-214

Aim of study (acc. to author)

To study the effects of 50 Hz magnetic fields with different magnetic flux densities on the cell nucleus area, some silver staining nucleolar organizer regions (AgNOR) parameters and the frequency of the micronucleated polychromatic erythrocytes and peripheral blood lymphocytes.

Background/further details

120 mice were divided into six groups (each group n=20) for the sham exposure and five different magnetic flux densities.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 40 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 40 days
Exposure setup
Exposure source
Setup six solenoids, each consisting of 300 turns of insulated copper wire wound in a single layer on a 32 cm diameter cylindrical plastic core; 10 animals of the same sex housed in one plastic cage; cage placed in the center of the solenoid
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 µT effective value calculated - 1, 2, 3, 4, 5 µT

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The extremely low frequency electromagnetic field exposure caused a nonlinear decline of the nucleus area compared with the sham exposed control group. A sharp drop occurred in AgNOR area of 1 µT group, and the data showed a regular increase with increased magnetic flux densities. The electromagnetic field did not change mean AgNOR numbers per nucleus of the groups. Relative AgNOR area had the highest level in 1 µT exposure group, and the level was quite similar to that of the 5 µT group. The remaining groups had significantly lower values quite similar to that of the control level.
The magnetic field exposure at any intensity did not affect significantly the frequency of the micronucleated blood cells. Nevertheless, micronucleus number per lymphocyte tended to increase at higher magnetic flux densities.
The results suggest that ≤ 5 µT intensities of 50 Hz magnetic fields did not cause genotoxic effects in the mouse.

Study character:

Study funded by

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