Study type: Medical/biological study (experimental study)

Effects on micronuclei formation of 60-Hz electromagnetic field exposure with ionizing radiation, hydrogen peroxide, or c-Myc overexpression. med./bio.

Published in: Int J Radiat Biol 2012; 88 (4): 374-380

Aim of study (acc. to author)

To study the combined effects of exposure to an extremely low frequency magnetic field and various stress factors including ionizing radiation, hydrogen peroxide, and oncogenic c-Myc activation on micronucleus formation in non-tumorigenic NIH3T3 cells and in human lung fibroblasts.

Background/further details

For the positive control, cells were exposed to various doses of gamma radiation (0-4 Gy) at a dose rate of 3.81 Gy/min. For H2O2 exposure, cells were treated with H2O2 (0-200 mM) for 4 h.
For the co-exposure experiments, there were three conditions: 1) cells exposed to an extremely low frequency magnetic field for 4 h, 2) cells exposed to an extremely low frequency magnetic field for 4 h immediately after irradiation with gamma rays (2 Gy), and 3) cells exposed to an extremely low frequency magnetic field for 4 h in the presence of H2O2 (100 µM).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 4 h

General information

Cells were exposed in the following eight groups: i) exposure to H2O2 ii) gamma radiation iii) exposure to ELF iv) sham exposure v) ELF exposure + exposure to H2O2 (100µM) vi) ELF exposure + gamma radiation (2 Gy) vii) c-Myc activation viii) ELF exposure + c-Myc activation

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 4 h
Exposure setup
Exposure source
Setup Merritt-coil system consisting of four square coils with 26 turns of wire in the outer and 11 turns in the inner coils, connected in series; cage with three testing floors (top, middle, bottom); exposure system shielded with ferrite material and placed inside a water-cooled incubator at a constant temperature of 37°C; cells placed in 60 mm Petri dishes inside the exposure system
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.01 mT - measured - -
magnetic flux density 0.5 mT - measured - -
magnetic flux density 1 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data did not show any significant differences between the cells exposed to extremely low frequency magnetic fields alone and the unexposed cells. Moreover, no synergistic effects were observed when extremely low frequency magnetic fields exposure was combined with ionizing radiation, hydrogen peroxide, and oncogenic c-Myc activation.
In conclusion, the findings demonstrate that extremely low frequency magnetic fields did not enhance micronucleus frequency caused by ionizing radiation, hydrogen peroxide, and oncogenic c-Myc activation.

Study character:

Study funded by

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