Study type: Medical/biological study (experimental study)

Effect of electromagnetic field on sertoli cell of rat testes: A light and transmission electron microscope study. med./bio.

Published in: JIMSA 2004; 17: 136-140

Aim of study (acc. to author)

To evaluate the effects of a prenatal and postnatal exposure to electromagnetic fields on the development of Sertoli cells in rats.

Background/further details

From 40 pregnant rats, 30 rats were selected for three weeks electromagnetic field exposure (intrauterine exposure) and ten rats as a control group. After delivery in both groups, 30 male pups each were exposed until five weeks of postnatal age. Subsequent to the exposure, 15 rats from both experimental (EG1) and control groups (CG1) were sacrificed. The remaining animals (EG2, CG2) were un-exposed for another eight weeks and then sacrificed.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 3 week in utero + 5 weeks after birth

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 3 week in utero + 5 weeks after birth
Exposure setup
Exposure source
Setup two coils separated by 50 cm; animals placed between the coils
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 8 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The experimental group 1 showed multiple features of disruption of normal Sertoli cell architecture: condensation of cell nucleus and presence of mitochondria without cristae, inclusion bodies, lysosomes and many vacuoles in the cytoplasm. Junctions between the Sertoli cells were destructed. In experimental group 2, there were signs of recovery in the Sertoli cells and spermatogonial cells following withdrawal from exposure. The empty spaces between the cells disappeared. The observations made for control group 2 were not different from control group 1.
The results suggest that long-term electromagnetic field exposure can cause profound changes in the Sertoli cells that may lead to sub-fertility.

Study character:

Study funded by

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