Study type: Medical/biological study (experimental study)

Effects of extremely low frequency electromagnetic field (ELF-EMF) on catalase, cytochrome P450 and nitric oxide synthase in erythro-leukemic cells. med./bio.

Published in: Life Sci 2015; 121: 117-123

Aim of study (acc. to author)

The effects of exposure of myelogenous leukemia cells to a 50 Hz magnetic field on enzymatic parameters for oxidative stress should be investigated.

Background/further details

Phorbol myristate acetate (PMA) was used as a positive control and in a co-exposure together with the magnetic field to stimulate cells and generate reactive oxygen species.
Cells were divided into 4 groups: 1) exposure to the magnetic field only, 2) exposure to PMA only, 3) PMA and magnetic field co-exposure and 4) control group.
Western blot analyses were conducted in triplicates in 4 different experiments.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for up to 24 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for up to 24 h
Exposure setup
Exposure source
Setup a 160 turn solenoid (22 cm in length, 6 cm in radius, copper wire diameter of 1.25 x 10-5 cm) placed inside an incubator generated a horizontal magnetic field in the same orientation as the geomagnetic field; field homogeneity at the location of cells was 98%; temperature was maintained at 37 ± 0.3°C and CO2 at 5%; temperature change in cell cultures was <0.1308°C
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -

Reference articles

  • Vianale G et al. (2008): Extremely low frequency electromagnetic field enhances human keratinocyte cell growth and decreases proinflammatory chemokine production.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

The total enzyme activity of catalase was significantly increased in all exposed cells (groups 1-3) compared to the control group and the peak time was significantly increased in PMA-exposed cells (group 2) and PMA + magnetic field co-exposed cells (group 3) in comparison to the control group. The catalase protein expression was significantly increased in PMA + magnetic field co-exposed cells compared to PMA-exposed cells.
The total enzyme activity and upward activity rate of cytochrome P450 were significantly increased in all exposed cells compared to the control group and the peak time was significantly increased in PMA-exposed cells and PMA + magnetic field co-exposed cells in comparison to the control group. The cytochrome P450 protein expression was significantly increased in magnetic field exposed cells (group 1) compared to the control group.
The total enzyme activity of the inducible nitric oxide synthase was significantly decreased in all exposed cells compared to the control group (remark EMF-Portal: the significance of further changes in the kinetic parameters of the inducible nitric oxide synthase was not specified clearly). The protein expression of the inducible nitric oxide synthase was significantly decreased in PMA + magnetic field co-exposed cells compared to PMA-exposed cells.
The authors conclude that exposure of myelogenous leukemia cells to a 50 Hz magnetic field could affect the oxidative equilibrium.

Study character:

Study funded by

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