Exposure duration: continuous for 4 h
|Exposure duration||continuous for 4 h|
|Chamber||exposure system was kept in an incubator at 37°C (±3°C); size of Petri dishes: 100 mm|
|Setup||field generator consisted of four square-shaped coils and one cage with three testing floors|
|Sham exposure||A sham exposure was conducted.|
|Additional info||magnetic field shielding system using ferrite material was adopted to shield strong magnetic fields in the outer of the exposure system|
|magnetic flux density||1 mT||maximum||measured and calculated||-||fixed value at the center of the middle floor|
The cells exposed to extremely low frequency magnetic fields did not show any statistically significant changes in the investigated parameters as compared to the sham exposed control. Gamma radiation exposed cells exhibited marked changes in reactive oxygen species level (increase), superoxide dismutase enzyme activity (increase), and GSH/GSSG ratio (decrease). Additionally, only the gamma radiation exposed cells were positive for morphological changes and senescence-associated beta-galactosidase activity.
The authors conclude that extremely low frequency magnetic field exposure has no effect on intracellular reactive oxygen species level, superoxide dismutase activity, and GSH/GSSG ratio under these exposure conditions.