Home
Medical/biological study (experimental study)

Short-term exposure to 50 Hz ELF-EMF alters the cisplatin-induced oxidative response in AT478 murine squamous cell carcinoma cells.

Published in: Bioelectromagnetics 2012; 33 (8): 641-651

Aim of study (acc. to author)

To study the influence of cisplatin and an extremely low frequency magnetic field on antioxidant enzyme activity and the lipid peroxidation, as well as on DNA damage and reactive oxygen species production in AT478 carcinoma cells.
Background/further details: The cells were divided into the following eight groups: 1) control group, incubated for 24 h, 2) control group, incubated for 72 h, 3) magnetic field exposure, incubated for 24 h, 4) magnetic field exposure, incubated for 72 h, 5) cisplatin treatment, incubated for 24 h, 6) cisplatin treatment, incubated for 72 h, 7) magnetic field exposure + cisplatin treatment, incubated for 24 h and 8) magnetic field exposure + cisplatin treatment, incubated for 72 h.
Additionally, for the comet assay cells were treated in the following three groups: 1) treatment with H2O2, 2) magnetic field exposure + treatment with H2O2 and 3) magnetic field exposure + cisplatin treatment (incubation for 24 h) + treatment with H2O2 for 5 min. The comet assay was performed 0, 60 and 120 min after removal of the H2O2 solution.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 16 min
General information
Cells were divided into the following eight groups: 1) control, collected after 24 h 2) control, collected after 72 h 3) exposure to EMF, collected after 24 h 4) exposure to EMF, collected after 72 h 5) treated with cisplatin, collected after 24 h 6) treated with cisplatin, collected after 72 h 7) exposure to EMF + treated with cisplatin, collected after 24 h 8) exposure to EMF + treated with cisplatin, collected after 72 h for comet assay cells were additionally treated in the following three groups: 1) treatment with H2O2 2) exposure to EMF + treatment with H2O2 3) exposure to EMF for 16 min + treatment with cisplatin for 24 h + treatment with H2O2 for 5 min
Exposure 1
Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 16 min
Exposure setup
Exposure source
Setup 40 cm long solenoid with a diameter of 20 cm consisting of 600 turns of 2 mm diamter copper wire wound in three forward-backward-forward continuous layers; solenoid placed in a water-jacketed, temperature and atmosphere controlled incubator; culture dishes inserted into a plexiglas cylinder and placed horizontally into the solenoid, so that the magnetic flux was parallel to the bottom
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -
Additional parameter details
local geomagnetic field: 37 µT AC background field: 2.09 µT
Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Cells treated with cisplatin alone exhibited a significant increase in reactive oxygen species and antioxidant enzyme activities. The magnetic field exposure + cisplatin treatment resulted in decreased reactive oxygen species levels and antioxidant enzyme activity. A significant reduction in malondialdehyde concentrations was observed in all of the study groups, with the greatest decrease associated with cisplatin treatment + magnetic field exposure.
Cisplatin induced the most severe DNA damage; however, when cells were also exposed to the magnetic field, less DNA damage occurred. Exposure to the magnetic field alone increased the DNA damage compared to the control cells.
The extremely low frequency magnetic field exposure decreased the effects of oxidative stress and DNA damage induced by cisplatin; however, the extremely low frequency magnetic field exposure alone was also a mild oxidative stressor and DNA damage inducer. The authors hypothesize that extremely low frequency magnetic field exposure exerts differential effects depending on the exogenous conditions.
Study character:

Study funded by

  • Medical University of Silesia, Poland

Related articles

  • Sanie-Jahromi F et al. (2016): Effects of extremely low frequency electromagnetic field and cisplatin on mRNA levels of some DNA repair genes.
  • Baharara J et al. (2016): Extremely low frequency electromagnetic field sensitizes cisplatin-resistant human ovarian adenocarcinoma cells via P53 activation.
  • Patruno A et al. (2015): Effects of extremely low frequency electromagnetic field (ELF-EMF) on catalase, cytochrome P450 and nitric oxide synthase in erythro-leukemic cells.
  • Yeganyan LR et al. (2012): Magnetically treated water at 4 Hz and 2.5 mT as a modulator of cisplatin effect on cell hydration and ouabain binding of sarcoma-180 tissue.
  • Chen WF et al. (2010): Static magnetic fields enhanced the potency of cisplatin on k562 cells.
  • Garip AI et al. (2010): Effect of ELF-EMF on number of apoptotic cells; correlation with reactive oxygen species and HSP.
  • Polaniak R et al. (2010): Influence of an Extremely Low Frequency Magnetic Field (ELF-EMF) on Antioxidative Vitamin E Properties in AT478 Murine Squamous Cell Carcinoma Culture In Vitro.
  • Villarini M et al. (2006): Effects of co-exposure to extremely low frequency (50 Hz) magnetic fields and xenobiotics determined in vitro by the alkaline comet assay.
  • Zwirska-Korczala K et al. (2005): Effect of extremely low frequency of electromagnetic fields on cell proliferation, antioxidative enzyme activities and lipid peroxidation in 3T3-L1 preadipocytes--an in vitro study.
  • Wolf FI et al. (2005): 50-Hz extremely low frequency electromagnetic fields enhance cell proliferation and DNA damage: possible involvement of a redox mechanism.
  • Zwirska-Korczala K et al. (2004): Influence of extremely-low-frequency magnetic field on antioxidative melatonin properties in AT478 murine squamous cell carcinoma culture.
  • Yoshizawa H et al. (2002): No effect of extremely low-frequency magnetic field observed on cell growth or initial response of cell proliferation in human cancer cell lines.
  • Ruiz-Gomez MJ et al. (2002): Influence of 1 and 25 Hz, 1.5 mT magnetic fields on antitumor drug potency in a human adenocarcinoma cell line.
  • Harris PA et al. (2002): Possible attenuation of the G2 DNA damage cell cycle checkpoint in HeLa cells by extremely low frequency (ELF) electromagnetic fields.
  • Loberg LI et al. (2000): Cell viability and growth in a battery of human breast cancer cell lines exposed to 60 Hz magnetic fields.