|Helmholtz coils with round pole pieces were located inside an incubator with 5% CO2, 95% humidity and 37.1 ± 0.1°C; cells were placed in the center between the coils
|control samples were placed into another incubator under the same physical conditions (temperature and humidity)
|magnetic flux density
Cells exposed to the static or 50 Hz magnetic field for 3 hours (groups 1 and 3) showed a significant increase in the intensity of the CH2 methylene group vibration bands compared to the control, which may be associated with an increase of cellular membrane surfaces due to extensive cellular growth. In addition, a decrease in the intensity of the PO2- phosphate bands was observed in both groups compared to the control, which might be related to an alteration in DNA/RNA (no further details shown in the article). An exposure to a static magnetic field for 18 hours (group 2) produced an increase of the beta sheet structure ratio and alpha helix structure ratio in the amide I region compared to the control due to unfolding processes in proteins (e.g. denaturation). Group 3 showed a significant increase of the beta sheet structure content in the amide I region, as well, and additionally a significant increase of the band assigned to non-hydrogen-bonded ester carbonyl stretching compared to the control, which may be an indication of cell death.
The authors conclude that the acute exposure of human neuroblastoma cells to a static or 50 Hz magnetic field might influence processes related to cell growth and that the exposure to a 50 Hz magnetic field might additionally influence cell death processes.