Study type: Medical/biological study (experimental study)

Static and 50 Hz Electromagnetic Fields Effects on Human Neuronal-Like Cells Vibration Bands in the Mid-Infrared Region. med./bio.

Published in: J Electromagn Anal 2011; 3 (2): 69-78

Aim of study (acc. to author)

The effects of an acute exposure of human neuroblastoma cells to a static or 50 Hz magnetic field on the ratio, conformation and derivatization of biomolecules should be investigated.

Background/further details

Cells were either exposed to a static magnetic field for 3 h (group 1) or 18 h (group 2), to a 50 Hz magnetic field for 3 h (group 3) or not exposed for control (group 4).

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuous for 3 or 18 h
Exposure 2: 50 Hz
Exposure duration: continuous for 3 h

Exposure 1

Main characteristics
Frequency
Type
Exposure duration continuous for 3 or 18 h
Exposure setup
Exposure source
Setup Helmholtz coils with round pole pieces were located inside an incubator with 5% CO2, 95% humidity and 37.1 ± 0.1°C; cells were placed in the center between the coils
Additional info control samples were placed into another incubator under the same physical conditions (temperature and humidity)
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 3 h
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Cells exposed to the static or 50 Hz magnetic field for 3 hours (groups 1 and 3) showed a significant increase in the intensity of the CH2 methylene group vibration bands compared to the control, which may be associated with an increase of cellular membrane surfaces due to extensive cellular growth. In addition, a decrease in the intensity of the PO2- phosphate bands was observed in both groups compared to the control, which might be related to an alteration in DNA/RNA (no further details shown in the article). An exposure to a static magnetic field for 18 hours (group 2) produced an increase of the beta sheet structure ratio and alpha helix structure ratio in the amide I region compared to the control due to unfolding processes in proteins (e.g. denaturation). Group 3 showed a significant increase of the beta sheet structure content in the amide I region, as well, and additionally a significant increase of the band assigned to non-hydrogen-bonded ester carbonyl stretching compared to the control, which may be an indication of cell death.
The authors conclude that the acute exposure of human neuroblastoma cells to a static or 50 Hz magnetic field might influence processes related to cell growth and that the exposure to a 50 Hz magnetic field might additionally influence cell death processes.

Study character:

Study funded by

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