Study type: Medical/biological study (experimental study)

Competition between hydrogen bonding and protein aggregation in neuronal-like cells under exposure to 50 Hz magnetic field. med./bio.

Published in: Int J Radiat Biol 2016; 92 (7): 395-403

Aim of study (acc. to author)

The effects of exposure of neuronal-like cells to a 50 Hz magnetic field on hydrogen bonds and protein unfolding should be investigated.

Background/further details

Hydrogen bonds play an important role in the formation and stabilization of the protein secondary structure. The delicate balance between many competing bonds can be altered by external stress, changing the structure of the protein.
12 samples were exposed to the magnetic field and the same number was used as the control group.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 4 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 4 hours
Exposure setup
Exposure source
Chamber 20 cm2 culture flasks between coils in incubator with 5% CO2 and 95% humidified air at 37°C
Setup two Helmholtz coils with pole pieces of round parallel polar faces; samples were placed at the center of a uniform field area between the coils; each coil had n=124 turns of wire and the coil spacing was assumed to be equal to the coils radius (r=150 mm); during the exposure, no significant increase in the temperature (± 0.1°C) was observed; magnetic field was perpendicular to the culture flask
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The amount of methylene groups was significantly increased in exposed cells compared to the control group. This might indicate an increase in cellular volume which would correspond to an increase in the surface area and the overall cellular content with fatty acids and phospholipids and a relative increase of methylene groups with respect to methyl groups.
Moreover, a significant decrease in primary amides and a significant increase of beta sheet structures in exposed cells compared to the control group indicated changes in the overall protein conformation state within the cell, which could be due to unfolding of proteins and formation of aggregates.
No significant changes were found for secondary amides.
The authors conclude that exposure to a 50 Hz magnetic field might increase both hydrogen bonds and protein unfolding processes in neuronal-like cells.

Study character:

Study funded by

Related articles