研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒトニューロン様細胞における中間赤外領域の振動バンドに対する静磁界および50Hz電磁界の影響] med./bio.

Static and 50 Hz Electromagnetic Fields Effects on Human Neuronal-Like Cells Vibration Bands in the Mid-Infrared Region.

掲載誌: J Electromagn Anal 2011; 3 (2): 69-78

この研究は、ヒトニューロン細胞静磁界(2mT)および50Hz磁界(1mT)を3時間ばく露した後、中間赤外領域のスペクトルのフーリエ自己デコンボリューション法による分析を行った。その結果、静磁界ばく露および50Hz磁界ばく露の後に、メチレン基の振動バンドが有意に増加した(細胞膜の構造変化に係わる脂質の増加を示唆する);リン酸基の伸縮振動バンドが減少した(DNA/RNAにおける変化を示唆する)、と報告している。

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研究目的(著者による)

The effects of an acute exposure of human neuroblastoma cells to a static or 50 Hz magnetic field on the ratio, conformation and derivatization of biomolecules should be investigated.

詳細情報

Cells were either exposed to a static magnetic field for 3 h (group 1) or 18 h (group 2), to a 50 Hz magnetic field for 3 h (group 3) or not exposed for control (group 4).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
  • DC/static
ばく露時間: continuous for 3 or 18 h
ばく露2: 50 Hz
ばく露時間: continuous for 3 h

ばく露1

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 continuous for 3 or 18 h
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 Helmholtz coils with round pole pieces were located inside an incubator with 5% CO2, 95% humidity and 37.1 ± 0.1°C; cells were placed in the center between the coils
Additional information control samples were placed into another incubator under the same physical conditions (temperature and humidity)
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 3 h
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
  • AC power
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Cells exposed to the static or 50 Hz magnetic field for 3 hours (groups 1 and 3) showed a significant increase in the intensity of the CH2 methylene group vibration bands compared to the control, which may be associated with an increase of cellular membrane surfaces due to extensive cellular growth. In addition, a decrease in the intensity of the PO2- phosphate bands was observed in both groups compared to the control, which might be related to an alteration in DNA/RNA (no further details shown in the article). An exposure to a static magnetic field for 18 hours (group 2) produced an increase of the beta sheet structure ratio and alpha helix structure ratio in the amide I region compared to the control due to unfolding processes in proteins (e.g. denaturation). Group 3 showed a significant increase of the beta sheet structure content in the amide I region, as well, and additionally a significant increase of the band assigned to non-hydrogen-bonded ester carbonyl stretching compared to the control, which may be an indication of cell death.
The authors conclude that the acute exposure of human neuroblastoma cells to a static or 50 Hz magnetic field might influence processes related to cell growth and that the exposure to a 50 Hz magnetic field might additionally influence cell death processes.

研究の種別:

研究助成

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