Study type: Medical/biological study (experimental study)

Co-exposure of ELF-magnetic fields and chemical mutagens: An investigation of genotoxicity with the SOS-based VITOTOX test in Salmonella typhimurium. med./bio.

Published in: Mutat Res Genet Toxicol Environ Mutagen 2016; 795: 31-35

Aim of study (acc. to author)

The genotoxic effects of exposure to a 50 Hz magnetic field alone and in combination with different chemical mutagens should be investigated in a bacterial assay.

Background/further details

Co-exposures to the magnetic field and the 4 following chemical mutagens with different modes of action were investigated: methyl methanesulfonate (0.1 mM and 1 mM), cis-platinum (0.01 and 0.1 mM), Aflatoxine B1 (0.1 and 1 µg/ml) and 2-amino-anthracene (0.25 and 2.5 µg/ml).
Tests were performed with and without addition of S9 mix (enzyme mix from rat liver) for metabolic activation.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 1 hour

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 1 hour
Exposure setup
Exposure source
Chamber microtiter plates
Setup exposures were performed in a cylindrical exposure unit (380-turn coil, 42 cm long, 20 cm inner diameter) at 37°C ± 0.5°C; the plates were placed in the middle of the coil
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - - - -

Reference articles

  • Verschaeve L et al. (2011): Genotoxicity investigation of ELF-magnetic fields in Salmonella typhimurium with the sensitive SOS-based VITOTOX test.
  • Maes A et al. (2000): Cytogenetic effects of 50 Hz magnetic fields of different magnetic flux densities.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposure to the magnetic field alone did not show any genotoxic effects and in co-exposure with different chemical mutagens, it did not change the genotoxic effects of the mutagens.
The authors conclude that exposure to a 50 Hz magnetic field has no genotoxic effects and does not influence genotoxicity induced by chemical mutagens in a bacterial assay.

Study character:

Study funded by

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