Study type: Medical/biological study (experimental study)

Genotoxicity investigation of ELF-magnetic fields in Salmonella typhimurium with the sensitive SOS-based VITOTOX test. med./bio.

Published in: Bioelectromagnetics 2011; 32 (7): 580-584

Aim of study (acc. to author)

To study the genotoxicity of extremely low frequency magnetic fields alone or in combination with known chemical mutagens using a sensitive test system (VITOTOX test) with Salmonella typhimurium.

Background/further details

The effect of magnetic field exposure alone and in combination with the three well-known mutagens 4-NQO (0.04 and 0.4 µg/ml), bleomycin (0.125 and 1.25 µg/ml) and benzpyrene (80 and 800 µg/ml), and the non-DNA-reactive chemical resorcinol (whose mutagenicity is questionable; 0.1 and 1 µg/ml) was investigated.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 1 or 2 h

General information

Bacteria were treated in ten groups: i) EMF exposure only ii) EMF exposure + 4-NQO iii) EMF exposure + bleomycin iv) EMF exposure + benzpyrene v) EMF exposure + resorcinol vi) 4-NQO only vii) bleomycin only viii) benzpyrene only ix) resorcinol only x) sham exposure

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 1 or 2 h
Exposure setup
Exposure source
Setup 42 cm long cylindrical coil with an inner diameter of 20 cm and 380 turns; bacteria cultures placed in 96-well plates in the middle of the coil
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - - - -
magnetic flux density 500 µT - - - -

Reference articles

  • Verheyen GR et al. (2003): Effect of coexposure to 50 Hz magnetic fields and an aneugen on human lymphocytes, determined by the cytokinesis block micronucleus assay.
  • Maes A et al. (2000): Cytogenetic effects of 50 Hz magnetic fields of different magnetic flux densities.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed that extremely low frequency magnetic fields did not induce SOS response-based mutagenicity in Salmonella typhimurium bacteria and did not show any synergistic effect when combined with chemical mutagens.

Study character:

Study funded by

Related articles