Study type: Medical/biological study (experimental study)

Cytogenetic effects of 50 Hz magnetic fields of different magnetic flux densities. med./bio.

Published in: Bioelectromagnetics 2000; 21 (8): 589-596

Aim of study (acc. to author)

This in vitro study was performed to investigate possible (co-)mutagen effects of extremely low frequency magnetic field in human blood lymphocytes and their dose dependent reponse.

Background/further details

The lymphocytes were isolated from the blood of 21 healthy male and female donors and they were exposed to magnetic field alone or in combination with the mutagen mitomycin C (0.1 µg/ml) or X-rays co-exposed.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: during entire cultivation time
EMF exposure only
Exposure 2: 50 Hz
Exposure duration: 30 min up to entire cultivation period
Exposure 3: 50 Hz
Exposure duration: entire cultivation period
EMF + X-rays; x-irradiation prior to cultivation
Exposure 4: 50 Hz
Exposure duration: 2 h continuous
EMF + X-rays; first exposure to EMF, then to X-rays, then 48 h cultivation

General information

Experiments were done in three groups: i) EMF exposure only ii) EMF exposure + Mitomycin C iii) EMF exposure + X-rays

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration during entire cultivation time
Additional info EMF exposure only
Exposure setup
Exposure source
  • coil(s)
  • cylindrical exposure unit: 42 cm long, 20 cm inner diameter
Setup 380 turns of 1 mm² wire
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2,500 µT maximum measured - 62.8, 80, 88.4, 504, 1061, 1750, 2500 µT

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration 30 min up to entire cultivation period
Additional info EMF exposure + MMC
Additional info the following combinations where examined (were x stands for the applied magnetic field strength): i) 0 µT + 0 µg/ml MMC (sham) ii) 0 µT + 0.1 µg/ml MMC (cells were exposed to MMC for 2 h prior to cell cultivation) iii) 0 µT + 0.1 µg/ml MMC (cells were exposed during the entire cultivation time) iv) x µT (30 min before cultivation) + 0 µg/ml MMC v) x µT (30 min before cultivation) + 0.1 µg/ml MMC (during cultivation) vi) x µT (during cultivation time) + 0 µg/ml MMC vii) x µT (during cultivation time) + 0.1 µg/ml MMC (only for 2 h before cultivation) viii) x µT + 0.1 µg/ml MMC (both during cultivation time)
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1,750 µT maximum measured - 88.4, 504, 1061, 1750 µT

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Exposure duration entire cultivation period
Additional info EMF + X-rays; x-irradiation prior to cultivation
Exposure setup
Exposure source
Additional info x-irradiation: 1 Gy per min
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1,750 µT maximum measured - 88.4, 504, 1061, 1750 µT

Exposure 4

Main characteristics
Frequency 50 Hz
Type
Exposure duration 2 h continuous
Additional info EMF + X-rays; first exposure to EMF, then to X-rays, then 48 h cultivation
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1,750 µT maximum measured - 80, 504, 1060, 1750 µT

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The exposure to extremely low frequency magnetic fields at seven different magnetic flux densities alone did not significantly influence the chromosome aberration and sister chromatid exchange frequency.
In general a co-mutagen effect was not observed in co-exposed cells. However, co-exposed cells (mitomycin C treated, at 504 µT) before and during cell cultivation showed a statistically significant decrease in sister chromatid exchange frequency. Also, when cells were cultivated in the presence of a magnetic field at 88.4 µT following X-ray exposure, there was a significant increase in chromosome aberration frequency compared to X-ray exposure alone.

Study character:

Study funded by

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