Study type: Medical/biological study (experimental study)

Pulsed electromagnetic fields promote survival and neuronal differentiation of human BM-MSCs. med./bio.

Published in: Life Sci 2016; 151: 130-138

Aim of study (acc. to author)

The effects of exposure of human bone marrow-derived mesenchymal stem cells to a pulsed 60 Hz magnetic field (PEMF) on cell viability and neuronal differentiation and underlying mechanisms of action should be investigated.

Background/further details

The study was conducted in view of a possible future use of PEMF in neurological diseases.
Cells were treated with differentiation medium (DMEM/F12 with 1 µM valproic acid, 2 µM forskolin, 2 µM butylated hydroxyanisole and 1 µM hydrocortisone) prior to exposure to the magnetic field.
Cells were also treated with the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 to investigate the effect of the magnetic field on protein kinase B pathway activation and regulation of BAD phosphorylation and with the inhibitor k252a of the TrkA receptor to investigate its involvement in signal transduction.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Modulation type: pulsed
Exposure duration: up to 24 hours

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
  • rectangular
Exposure duration up to 24 hours
Modulation
Modulation type pulsed
Exposure setup
Exposure source
Chamber cell culture incubator at 5% CO2 and 37°C
Setup Helmholtz coils (15 inner diameter) in incubator
Sham exposure A sham exposure was conducted.
Additional info (remark EMF-Portal: results are given for exposure durations up to 24 hours only though longer exposure durations are mentioned in the methods)
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 10 mT - measured - -

Reference articles

  • Cho H et al. (2012): Neural stimulation on human bone marrow-derived mesenchymal stem cells by extremely low frequency electromagnetic fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Cell viability and the ratio of living/apoptotic cells were significantly increased after 24 h of exposure to the magnetic field compared to the control group.
The gene expressions and/or protein expressions of neural markers NF-L, NeuroD1, Tau and MAP2 were significantly increased in exposed cells compared to the control group.
Exposure to the magnetic field also significantly increased the activity of protein kinase B (Akt), Rsk, CREB, ERK, Bcl-xL and BAD via phosphorylation. Pretreatment with the PI3K inhibitor LY294002 reversed the cell viability-enhancing effect of the magnetic field.
These results suggested that the cell-protective effect of PEMF was mediated by the PI3K/Akt/BAD pathway and that the neuronal differentiating effect was based on the CREB/ERK/Rsk pathway. The TrkA receptor can activate both signal pathways and there was a significantly increased TrkA protein expression in PEMF-exposed cells compared to the control group. When using the TrkA inhibitor K252a in PEMF exposed cells, the protein expression of phosphorylated protein kinase B (Akt) was also decreased, indicating an association.
The authors conclude that exposure of human bone marrow-derived mesenchymal stem cells to a pulsed 60 Hz magnetic field might enhance cell survival and induce neuronal differentiation via activation of the TrkA receptor and two different signal pathways. This result might be beneficial for future work on cell transplantation therapy for neurological diseases.

Study character:

Study funded by

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