研究のタイプ: 医学/生物学の研究 (experimental study)

[パルス化電磁界はヒトBM-MSCの生存および神経分化を促進する] med./bio.

Pulsed electromagnetic fields promote survival and neuronal differentiation of human BM-MSCs.

掲載誌: Life Sci 2016; 151: 130-138

この研究は、パルス電磁界PEMFばく露によりヒトBM-MSC細胞株アポトーシス抑制および生存促進神経分化を含む)が見られるか否かを検討した。その結果、PEMF処置により、神経マーカ(NF-L、NeuroD1、タウなど)の発現増加し、ばく露量およびばく露時間に依存して細胞死が大きく低下することが観察されたとして、このような細胞保護効果のメカニズムにするかも知れない信号伝達経路に関する分析の結果も報告している。

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研究目的(著者による)

The effects of exposure of human bone marrow-derived mesenchymal stem cells to a pulsed 60 Hz magnetic field (PEMF) on cell viability and neuronal differentiation and underlying mechanisms of action should be investigated.

詳細情報

The study was conducted in view of a possible future use of PEMF in neurological diseases.
Cells were treated with differentiation medium (DMEM/F12 with 1 µM valproic acid, 2 µM forskolin, 2 µM butylated hydroxyanisole and 1 µM hydrocortisone) prior to exposure to the magnetic field.
Cells were also treated with the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 to investigate the effect of the magnetic field on protein kinase B pathway activation and regulation of BAD phosphorylation and with the inhibitor k252a of the TrkA receptor to investigate its involvement in signal transduction.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
Modulation type: pulsed
ばく露時間: up to 24 hours

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
波形
  • rectangular
ばく露時間 up to 24 hours
Modulation
Modulation type pulsed
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cell culture incubator at 5% CO2 and 37°C
ばく露装置の詳細 Helmholtz coils (15 inner diameter) in incubator
Sham exposure A sham exposure was conducted.
Additional information (remark EMF-Portal: results are given for exposure durations up to 24 hours only though longer exposure durations are mentioned in the methods)
パラメータ
測定量 種別 Method Mass 備考
磁束密度 10 mT - 測定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Cell viability and the ratio of living/apoptotic cells were significantly increased after 24 h of exposure to the magnetic field compared to the control group.
The gene expressions and/or protein expressions of neural markers NF-L, NeuroD1, Tau and MAP2 were significantly increased in exposed cells compared to the control group.
Exposure to the magnetic field also significantly increased the activity of protein kinase B (Akt), Rsk, CREB, ERK, Bcl-xL and BAD via phosphorylation. Pretreatment with the PI3K inhibitor LY294002 reversed the cell viability-enhancing effect of the magnetic field.
These results suggested that the cell-protective effect of PEMF was mediated by the PI3K/Akt/BAD pathway and that the neuronal differentiating effect was based on the CREB/ERK/Rsk pathway. The TrkA receptor can activate both signal pathways and there was a significantly increased TrkA protein expression in PEMF-exposed cells compared to the control group. When using the TrkA inhibitor K252a in PEMF exposed cells, the protein expression of phosphorylated protein kinase B (Akt) was also decreased, indicating an association.
The authors conclude that exposure of human bone marrow-derived mesenchymal stem cells to a pulsed 60 Hz magnetic field might enhance cell survival and induce neuronal differentiation via activation of the TrkA receptor and two different signal pathways. This result might be beneficial for future work on cell transplantation therapy for neurological diseases.

研究の種別:

研究助成

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