Study type: Medical/biological study (experimental study)

Effect of electromagnetic fields on proliferation and differentiation of cultured mouse bone marrow mesenchymal stem cells. med./bio.

Published in: J Huazhong Univ Sci Technolog Med Sci 2005; 25 (2): 185-187

Aim of study (acc. to author)

To study the effects of electromagnetic fields on proliferation, differentiation and intercellular cyclic AMP in mouse bone marrow mesenchymal stem cells in vitro.

Background/further details

The mouse bone marrow mesenchymal stem cells were divided into four groups: Group A was the sham exposure group, group B was exposed twice every day (each 60 min) with a time interval of 6 h. The cells of group C and D contained osteogenesis inducer. The cells of group C were not exposed to electromagnetic fields; the cells of group D were exposed to electromagnetic fields as the same as group B. The cellular proliferation, the cellular differentiation and the intercellular cAMP level were investiagted at different time points (during exposure).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 60 min, twice a day with an interval of 6 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 60 min, twice a day with an interval of 6 h
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT peak value - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure

Main outcome of study (acc. to author)

The exposure to electromagnetic fields inhibited the cellular proliferation, enhanced the cellular differentiation and increased the intercellular cAMP level in the early time of the exposure (1-3 days).The intercellular cAMP level did not increase further in the later days.
The authors conclude that the cAMP may be involved in the mediation of the growth inhibitory and differentiation-inducing signals of specific electromagnetic fields in vitro.

Study character:

Study funded by

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