Extremely low-frequency electromagnetic fields affect transcript levels of neuronal differentiation-related genes in embryonic neural stem cells.
Published in: PLoS One 2014; 9 (3): e90041
Aim of study (acc. to author)
cells were exposed in plates with 96 wells ( cell viability) or in 35 mm Petri dishes
exposure system was composed of two four- coil systems (two coils with 56 windings, two coils with 50 windings) and placed inside a µ-metal chamber; both chambers were placed inside an incubator (37°C ± 0.5°C, while temperature variance between the chambers did not exceed 0.3°C; 5% CO 2; 95% humidity)
sham exposure was conducted.
molecular biosynthesis: DNA synthesis (EdU incorporation, fluorescence microscopy); gene expression of cell cycle related genes ( p53, p21, and GADD45) and different markers for cell differentiation (Tuj1, GFAP, Sox2, Math1, Math3, Ngn1, Ngn2, Hes6, NeuroD, Hes1, Hes5) ( Real-time PCR)
cell viability/ cell division/ proliferation: cell proliferation (via cell viability, commercial cell counting kit, spectrophotometry); cell cycle distribution ( propidium iodide staining, flow cytometry); cell differentiation ( protein expression of Tuj1 ( marker for neurons) and GFAP ( marker for astrocytes), immunocytochemistry, fluorescence microscopy) diameter of neurospheres (
culture system composed of clusters of neural stem cells) Main outcome of study (acc. to author)
Study funded by
National Basic Research Program (Program 973), China
National Natural Science Foundation (NSFC), China
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