研究のタイプ: 医学／生物学の研究 (experimental study)

[50Hz磁界ばく露下の神経様細胞における水素結合とタンパク質凝集の競合] med./bio.

Competition between hydrogen bonding and protein aggregation in neuronal-like cells under exposure to 50 Hz magnetic field

著者: Calabro E
掲載誌: Int J Radiat Biol 2016; 92 (7): 395-403
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この研究は、磁界（50Hz、1mT）の4時間ばく露がヒトSH-SY5Y細胞の水素結合およびタンパク質高次構造に与える影響を調べた。ばく露システムは2個のヘルムホルツコイルで構成された。ばく露後に試料をフーリエ変換赤外分光（FTIR）分析した。結果を総合すると、50 Hz磁界ばく露下でタンパク質凝集と水素結合増加の両方のメカニズムが働いたと推測される、と報告している。

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研究目的（著者による）

The effects of exposure of neuronal-like cells to a 50 Hz magnetic field on hydrogen bonds and protein unfolding should be investigated.

詳細情報

Hydrogen bonds play an important role in the formation and stabilization of the protein secondary structure. The delicate balance between many competing bonds can be altered by external stress, changing the structure of the protein.
12 samples were exposed to the magnetic field and the same number was used as the control group.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 4 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 hours
ばく露装置
ばく露の発生源／構造
チャンバの詳細 20 cm2 culture flasks between coils in incubator with 5% CO2 and 95% humidified air at 37°C
ばく露装置の詳細 two Helmholtz coils with pole pieces of round parallel polar faces; samples were placed at the center of a uniform field area between the coils; each coil had n=124 turns of wire and the coil spacing was assumed to be equal to the coils radius (r=150 mm); during the exposure, no significant increase in the temperature (± 0.1°C) was observed; magnetic field was perpendicular to the culture flask
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -
Additional parameter details

H field within the volume of exposed culture was ± 0.01 mT during exposure; H field from incubators in the area where samples were located was 0.002 mT

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The amount of methylene groups was significantly increased in exposed cells compared to the control group. This might indicate an increase in cellular volume which would correspond to an increase in the surface area and the overall cellular content with fatty acids and phospholipids and a relative increase of methylene groups with respect to methyl groups.
Moreover, a significant decrease in primary amides and a significant increase of beta sheet structures in exposed cells compared to the control group indicated changes in the overall protein conformation state within the cell, which could be due to unfolding of proteins and formation of aggregates.
No significant changes were found for secondary amides.
The authors conclude that exposure to a 50 Hz magnetic field might increase both hydrogen bonds and protein unfolding processes in neuronal-like cells.

研究の種別:

研究助成

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