Study type: Medical/biological study (experimental study)

Effects of combined radiofrequency field exposure on amyloid-beta-induced cytotoxicity in HT22 mouse hippocampal neurones. med./bio.

Published in: J Radiat Res 2016; 57 (6): 620-626

Aim of study (acc. to author)

The effects of exposure of mouse hippocampal neurons to combined 837 MHz and 1950 MHz electromagnetic fields on beta-amyloid protein-induced cytotoxicity should be investigated.

Background/further details

Few previous animal studies suggested beneficial effect of radiofrequency fields in Alzheimer's disease (Arendash et al. 2010, Banaceur et al. 2013). In contrast, EMF exposure was also found to increase beta-amyloid protein expression (Jiang et al. 2013). The present study should elucidate the possible mechanism of action in vitro.
Cells were divided into the following groups: 1) exposure to the electromagnetic fields only, 2) exposure to the electromagnetic fields and treatment with 5 µM beta-amyloid protein, 3) exposure to the electromagnetic fields and treatment with 50 µM beta-amyloid protein. For each group, a separate sham exposure group was used.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 837–1,950 MHz
Exposure duration: continuous for 2 hours

General information

cells were treated with respective concentrations of beta-amyloid protein (5 µM and 50 µM) for 30 minutes prior to exposure to the electromagnetic fields

Exposure 1

Main characteristics
Frequency 837–1,950 MHz
Type
Exposure duration continuous for 2 hours
Modulation
Modulation type cf. additional info
Additional info

simultaneous CDMA signal at 837 MHz and W-CDMA signal at 1950 MHz

Exposure setup
Exposure source
Distance between exposed object and exposure source 13.6 cm
Chamber 100 mm petri dishes in exposure chamber with 37 ± 0.3°C and a 5% CO2
Setup radial transmission line (RTL) exposure system with conical antenna, which was located in the center of the exposure chamber; petri dishes were placed at 13.6 cm from the antenna
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg - calculated - CDMA signal
SAR 2 W/kg - calculated - W-CDMA signal

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposure to the electromagnetic fields had no significant effect on any parameter in any group compared to the respective sham exposure groups.
The authors conclude that exposure of mouse hippocampal neurons to combined 837 MHz and 1950 MHz electromagnetic fields has no effects on the beta-amyloid protein-induced cytotoxicity.

Study character:

Study funded by

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