40 mice were mated and on the following day, zygotes were isolated and incubated for 5 hours. Afterwards, 2-cell-state embryos were divided into 2 groups (n=150 each): exposure group and control group.
|Exposure duration||30 minutes/day for 4 days|
|Chamber||incubator with 37°C and 5% CO2|
|Setup||"cell phone was held horizontally and parallel to the zygotes culture medium inside the incubator while the bottom of the phone being placed 1 cm above the upper from the stage of incubator" (?); phone was kept in talk mode and the distance between the phone and embryos was > 10 cm|
|Additional info||embryos of the control group were kept in an incubator without exposure to the cell phone|
At the 2-cell stage, the percentage of dead embryos was significantly increased in the exposure group compared to the control group. Moreover, the rate of dead cells was significantly increased in blastocysts from the exposure group compared to the control group.
The embryonal survival rate at the blastocyst stage did not show significant differences between both groups.
The authors conclude that exposure of mice 2-cell-state embryos to a 900-1800 MHz electromagnetic field has no detrimental effects on embryonal development but might decrease cell viability.