Sperm cells and oocytes were collected from mice and separately exposed or sham exposed for 1 hour, respectively. Afterwards, an in vitro fertilization was performed. Thereby, 4 groups were formed: 1a) exposed sperm cells + exposed oocytes, 2a) exposed sperm cells + sham exposed oocytes, 3a) sham exposed sperm cells + exposed oocytes and 4a) sham exposed sperm cells and sham exposed oocytes. As an alternative fertilization method, "intra-cytoplasmic sperm injection" was used and the same groups were examined (groups 1b, 2b, 3b and 4b).
Exposure duration: 60 minutes
|Exposure duration||60 minutes|
|Chamber||sperm cells and oocytes were respectively exposed in 35-mm Petri dishes|
|Setup||exposure system was put in an incubator (37°C, 5% CO2, 100% humidity) and consisted of two RF-generators each of which was included into a separate waveguide together with frames on which the dishes for exposure were mounted; temperature change in the culture medium due to exposure was 0.13°C/W/kg or less|
|Sham exposure||A sham exposure was conducted.|
|Additional info||the non-uniformity in SAR distribution for the medium for spermatozoa in 35- mm Petri dishes was 2.4 dB, whereas that in the medium for oocytes was 1.4 dB|
No significant differences in the fertilization rate, the number of embryos and chromosome aberrations were found between any of the groups.
The authors conclude that exposure to a radiofrequency electromagnetic field had no influence on fertilization and embryogenesis of mice in vitro. Furthermore, they conclude that the results indicate safety of radiofrequency electromagnetic fields in humans, considering that the exposure in the present study was 100 times greater for sperm cells and oocytes than in real life.