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Medical/biological study

Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line.

Published in: J Lasers Med Sci 2017; 8 (2): 79-83
Journal not peer-reviewed

Aim of study (acc. to author)

To investigate the effects of 50 Hz magnetic fields on cell morphology and protein expression in a human neuroblastoma cell line.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 3 hours
Exposure 2: 50 Hz
Exposure duration: 3 hours
Exposure 1
Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 3 hours
Exposure setup
Exposure source
Setup Helmholtz coil was put in an incubator (37°C, 5% CO2); control group were put in the same incubator, but outside the coil
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.5 mT - - - -
Exposure 2
Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 3 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - - - -
Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:

Main outcome of study (acc. to author)

In exposed cell cultures, cell proliferation was decreased compared to the control group (remark EMF-Portal: not clear if signifcant). Additionally, the cell morphology was changed in exposed cells (remark EMF-Portal: no further details given). After exposure to a magnetic field of 0.5 mT/1 mT, the expression of 64/86 proteins was significantly up-regulated and the expression of 40/65 proteins was significantly down-regulated compared to the control group.
The authors conclude that 50 Hz magnetic fields could lead to alterations in cell morphology and protein expression in a human neuroblastoma cell line in a dose dependent manner.
Study character:

Study funded by

  • Shahid-Beheshti University (SBU), Tehran, Iran
  • Semnan University of Medical Sciences, Iran

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  • Luukkonen J et al. (2017): Modification of p21 level and cell cycle distribution by 50 Hz magnetic fields in human SH-SY5Y neuroblastoma cells.
  • Reale M et al. (2016): Effect of environmental extremely low-frequency electromagnetic fields exposure on inflammatory mediators and serotonin metabolism in a human neuroblastoma cell line.
  • Akbarnejad Z et al. (2016): Effects of extremely low-frequency pulsed electromagnetic fields (ELF-PEMFs) on glioblastoma cells (U87).
  • Falone S et al. (2016): Improved Mitochondrial and Methylglyoxal-Related Metabolisms Support Hyperproliferation Induced by 50 Hz Magnetic Field in Neuroblastoma Cells.
  • Hasanzadeh H et al. (2014): Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis.
  • Kanitz MH et al. (2007): Investigation of protein expression in magnetic field-treated human glioma cells.
  • Savage Jr RE et al. (2005): Changes in gene and protein expression in magnetic field-treated human glioma cells.