Medical/biological study

Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line med./bio.

By: Rezaie-Tavirani M, Hasanzadeh H, Seyyedi S, Zali H

Published in: J Lasers Med Sci 2017; 8 (2): 79-83

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: 3 hours	magnetic flux density: 0.5 mT
Exposure 2: 50 Hz Exposure duration: 3 hours	magnetic flux density: 1 mT

Exposure source	Helmholtz coils
Setup	Helmholtz coil was put in an incubator (37°C, 5% CO₂); control group were put in the same incubator, but outside the coil
Sham exposure	A sham exposure was conducted.

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	0.5 mT	-	-	-	-

Exposure source	same setup as exposure 1
Sham exposure	A sham exposure was conducted.

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	1 mT	-	-	-	-

Exposed system:

Investigated system:

In exposed cell cultures, cell proliferation was decreased compared to the control group (remark EMF-Portal: not clear if signifcant). Additionally, the cell morphology was changed in exposed cells (remark EMF-Portal: no further details given). After exposure to a magnetic field of 0.5 mT/1 mT, the expression of 64/86 proteins was significantly up-regulated and the expression of 40/65 proteins was significantly down-regulated compared to the control group.
The authors conclude that 50 Hz magnetic fields could lead to alterations in cell morphology and protein expression in a human neuroblastoma cell line in a dose dependent manner.

Study character:

Akbarnejad Z et al. (2017): Effects of extremely low-frequency pulsed electromagnetic fields (ELF-PEMFs) on glioblastoma cells (U87)
Luukkonen J et al. (2017): Modification of p21 level and cell cycle distribution by 50 Hz magnetic fields in human SH-SY5Y neuroblastoma cells
Falone S et al. (2017): Power frequency magnetic field promotes a more malignant phenotype in neuroblastoma cells via redox-related mechanisms
Falone S et al. (2016): Improved Mitochondrial and Methylglyoxal-Related Metabolisms Support Hyperproliferation Induced by 50 Hz Magnetic Field in Neuroblastoma Cells
Reale M et al. (2016): Effect of environmental extremely low-frequency electromagnetic fields exposure on inflammatory mediators and serotonin metabolism in a human neuroblastoma cell line
Hasanzadeh H et al. (2014): Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis
Kanitz MH et al. (2007): Investigation of protein expression in magnetic field-treated human glioma cells
Savage Jr RE et al. (2005): Changes in gene and protein expression in magnetic field-treated human glioma cells