Study type: Medical/biological study

Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line. med./bio.

Published in: J Lasers Med Sci 2017; 8 (2): 79-83
Journal not peer-reviewed

Aim of study (acc. to author)

To investigate the effects of 50 Hz magnetic fields on cell morphology and protein expression in a human neuroblastoma cell line.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 3 hours
Exposure 2: 50 Hz
Exposure duration: 3 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 3 hours
Exposure setup
Exposure source
Setup Helmholtz coil was put in an incubator (37°C, 5% CO2); control group were put in the same incubator, but outside the coil
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.5 mT - - - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 3 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:

Main outcome of study (acc. to author)

In exposed cell cultures, cell proliferation was decreased compared to the control group (remark EMF-Portal: not clear if signifcant). Additionally, the cell morphology was changed in exposed cells (remark EMF-Portal: no further details given). After exposure to a magnetic field of 0.5 mT/1 mT, the expression of 64/86 proteins was significantly up-regulated and the expression of 40/65 proteins was significantly down-regulated compared to the control group.
The authors conclude that 50 Hz magnetic fields could lead to alterations in cell morphology and protein expression in a human neuroblastoma cell line in a dose dependent manner.

Study character:

Study funded by

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