Study type: Medical/biological study (experimental study)

Extremely low-frequency electromagnetic field influences the survival and proliferation effect of human adipose derived stem cells. med./bio.

Published in: Adv Biomed Res 2014; 3: 25-

Aim of study (acc. to author)

To investigate the effects of extremely low frequency magnetic fields on the proliferation and cell viability of human adipose derived stem cells.

Background/further details

Cells were exposed to magnetic field intensities of 0.5 mT or 1 mT. Exposure duration was 20 or 40 minutes per day.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 20 or 40 minutes per day for 7 days
Exposure 2: 50 Hz
Exposure duration: 20 or 40 minutes per day for 7 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 20 or 40 minutes per day for 7 days
Exposure setup
Exposure source
Chamber cells were cultured and exposed in 96 well plates
Setup solenoid consisted of 720 turns of 1 mm enamel copper wire and was wound on a cylindrical core of acrylic tube (inner diameter 20 cm, height 24 cm); solenoid was connected to an autotransformer which was connected to 220 V power; uniformity of magnetic field at the center of solenoid was ± 1% where cultures were located
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.5 mT - measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 20 or 40 minutes per day for 7 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The cell proliferation and cell viability were significantly increased in cell cultures exposed to a magnetic field intensity of 1 mT for 20 minutes and of 0.5 mT for 20 and 40 minutes compared to the sham exposure.
The authors conclude that exposure to extremely low frequency magnetic fields could enhance the cell viability and cell proliferation of human adipose derived stem cells.

Study character:

Study funded by

Related articles