Due to a lack of financial resources, we unfortunately have to suspend the import of any new radio frequency and mobile phone-related articles as of now (November 27, 2017). We apologize for this inconvenience and will keep you informed.
Home
Medical/biological study (experimental study)

No evidence of DNA damage by co-exposure to extremely low frequency magnetic fields and aluminum on neuroblastoma cell lines.

Published in: Mutation Research - Fundamental and Molecular Mechanism of Mutagenesis 2017; 823: 11-21

Aim of study (acc. to author)

To investigate the effects of co-exposure to a 50 Hz magnetic field and aluminium on DNA damage in neuroblastoma cell lines.
Background/further details: In a pre-test, aluminium chloride concentrations above 40 µM were found to be cytotoxic. Therefore, concentrations up to this value were used in the experiments.
Four groups were examined for every cell line, respectively: 1) control group, 2) exposure to aluminium chloride (4 or 40 µM AlCl3), 3) 50 Hz magnetic field exposure (0.01 mT, 0.1 mT, or 1 mT) and 4) co-exposure to aluminium chloride (4 or 40 µM AlCl3) and 50 Hz magnetic field (0.01 mT, 0.1 mT, or 1 mT).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 1 hour (continuously)
Exposure 2: 50 Hz
Exposure duration: 5 hours (intermittent, i.e. 15 min on, 15 min off)
Exposure 1
Main characteristics
Frequency 50 Hz
Type
Exposure duration 1 hour (continuously)
Exposure setup
Exposure source
Chamber cells were exposed in 6-well plates
Setup magnetic field was generated by an apparatus consisting of a pair of parallel coils (16.5 cm diameter (external) and 12 cm distance between the coils) arranged horizontally in a Helmholtz-configuration; well plates were positioned at the center of the coil system wit 2% field uniformity; coil system was put into a standard CO2 incubator; temperature was 37.0°C ± 0.3°C
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.01 mT - measured - -
magnetic flux density 0.1 mT - measured - -
magnetic flux density 1 mT - measured - -
Exposure 2
Main characteristics
Frequency 50 Hz
Type
Exposure duration 5 hours (intermittent, i.e. 15 min on, 15 min off)
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.01 mT - measured - -
magnetic flux density 0.1 mT - measured - -
magnetic flux density 1 mT - measured - -
Reference articles
  • Moretti M et al. (2005): Effects of co-exposure to extremely low frequency (ELF) magnetic fields and benzene or benzene metabolites determined in vitro by the alkaline comet assay.
Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant differences in exposed or co-exposed cells were found in any of the examined parameters compared to the control group.
The authors conclude that neither short-term exposure to aluminium chloride or to a 50 Hz magnetic field alone nor a combination of both has any detrimental effect on human neuroblastoma cells.
Study character:

Study funded by

  • Fondazione Cassa di Risparmio di Perugia, Italy
  • University of Perugia, Italy

Related articles

  • Falone S et al. (2017): Power frequency magnetic field promotes a more malignant phenotype in neuroblastoma cells via redox-related mechanisms.
  • Luukkonen J et al. (2017): Modification of p21 level and cell cycle distribution by 50 Hz magnetic fields in human SH-SY5Y neuroblastoma cells.
  • Benassi B et al. (2016): Extremely low frequency magnetic field (ELF-MF) exposure sensitizes SH-SY5Y cells to the pro-Parkinson's disease toxin MPP+.
  • Kesari KK et al. (2016): Induction of micronuclei and superoxide production in neuroblastoma and glioma cell lines exposed to weak 50 Hz magnetic fields.
  • Luukkonen J et al. (2014): Induction of genomic instability, oxidative processes, and mitochondrial activity by 50Hz magnetic fields in human SH-SY5Y neuroblastoma cells.
  • Luukkonen J et al. (2011): Pre-Exposure to 50 Hz Magnetic Fields Modifies Menadione-Induced Genotoxic Effects in Human SH-SY5Y Neuroblastoma Cells.
  • Villarini M et al. (2006): Effects of co-exposure to extremely low frequency (50 Hz) magnetic fields and xenobiotics determined in vitro by the alkaline comet assay.
  • Moretti M et al. (2005): Effects of co-exposure to extremely low frequency (ELF) magnetic fields and benzene or benzene metabolites determined in vitro by the alkaline comet assay.
  • Cho YH et al. (2003): The effect of extremely low frequency electromagnetic fields (ELF-EMF) on the frequency of micronuclei and sister chromatid exchange in human lymphocytes induced by benzo(a)pyrene.
  • Suri A et al. (1996): A 3 milliTesla 60 Hz magnetic field is neither mutagenic nor co-mutagenic in the presence of menadione and MNU in a transgenic rat cell line.