In a pre-test, aluminium chloride concentrations above 40 µM were found to be cytotoxic. Therefore, concentrations up to this value were used in the experiments.
Four groups were examined for every cell line, respectively: 1) control group, 2) exposure to aluminium chloride (4 or 40 µM AlCl3), 3) 50 Hz magnetic field exposure (0.01 mT, 0.1 mT, or 1 mT) and 4) co-exposure to aluminium chloride (4 or 40 µM AlCl3) and 50 Hz magnetic field (0.01 mT, 0.1 mT, or 1 mT).
Exposure duration: 1 hour (continuously)
Exposure duration: 5 hours (intermittent, i.e. 15 min on, 15 min off)
|Exposure duration||1 hour (continuously)|
|Chamber||cells were exposed in 6-well plates|
|Setup||magnetic field was generated by an apparatus consisting of a pair of parallel coils (16.5 cm diameter (external) and 12 cm distance between the coils) arranged horizontally in a Helmholtz-configuration; well plates were positioned at the center of the coil system wit 2% field uniformity; coil system was put into a standard CO2 incubator; temperature was 37.0°C ± 0.3°C|
No significant differences in exposed or co-exposed cells were found in any of the examined parameters compared to the control group.
The authors conclude that neither short-term exposure to aluminium chloride or to a 50 Hz magnetic field alone nor a combination of both has any detrimental effect on human neuroblastoma cells.